摘要
Conductive polymers show great promise because of their electrical property based on bioelectricity in vivo. In order to search electro-activity materials which insure abduction of tissue, we synthesized conductive nerve conduits with poly-dl-lactic-acid(PDLLA) and tetra-aniline(TA). Preparation technology of TA was optimized, and the properties of the conduit were studied. RSC96 cell were used to investigate the toxicity and electrical stimulation effect. SD rats were used to assess biocompatibility in vivo. The results showed that the reaction ratio of 1:1, the reaction time of 2 h and the HCl concentration of 2 mol/L were the optimum conditions for synthesis of TA. The influence of TA content on the mechanical properties, hydrophilicity, conductivity and microstructure of the nerve conduit was evaluated. Cell and histocompatibility study showed PDLLA/TA possessed good biocompatibility. These results showed it had application values in tissue projects.
Conductive polymers show great promise because of their electrical property based on bioelectricity in vivo. In order to search electro-activity materials which insure abduction of tissue, we synthesized conductive nerve conduits with poly-dl-lactic-acid(PDLLA) and tetra-aniline(TA). Preparation technology of TA was optimized, and the properties of the conduit were studied. RSC96 cell were used to investigate the toxicity and electrical stimulation effect. SD rats were used to assess biocompatibility in vivo. The results showed that the reaction ratio of 1:1, the reaction time of 2 h and the HCl concentration of 2 mol/L were the optimum conditions for synthesis of TA. The influence of TA content on the mechanical properties, hydrophilicity, conductivity and microstructure of the nerve conduit was evaluated. Cell and histocompatibility study showed PDLLA/TA possessed good biocompatibility. These results showed it had application values in tissue projects.
基金
Funded by the National Natural Science Foundation of China(Nos.51473130 and 51572206)
the Wuhan Huanghe Excellence Plan and Entrepreneurship Training Program of Wuhan University of Technology(Nos.20181049715010 and 20181049715018)
