植物激素及遮阳处理对铁皮石斛多糖含量的影响

Effects of Phytohormones and Sunshading on Polysaccharide Content in Dendrobii officinalis

本研究以对不同植物激素及遮阳处理对不同年份广西产铁皮石斛的多糖含量进行比较分析,为广西产铁皮石斛栽培提供科学依据。实验中参考2015年版《中国药典》的方法,采用UV-VIS对不同实验组的广西产铁皮石斛进行多糖含量测定,利用Mintab17软件进行差异性分析。研究结果表明:以GA3处理组的铁皮石斛多糖含量为最高,多糖含量最高的为GA3处理组的一年生盆枝条多糖含量高可达44.01%;遮阳处理能提高多年生铁皮石斛的多年生枝条的多糖含量;而6-BA处理组的多糖含量显著低于其他处理实验组。本研究初步表明,GA3处理能有效提高铁皮石斛的多糖含量,这对加强铁皮石斛的中药功能具有重要作用。

This study compared and analyzed the polysaccharide content of Dendrobium candidum produced in Guangxi in different years with different plant hormones and sunshading treatments, providing scientific basis for the cultivation of Dendrobium candidum. Based on the method of Chinese Pharmacopoeia (2015), the polysaccharide content of Dendrobii officinalis of different experimental groups was determined by UV-VIS, and the difference was analyzed by the software of Mintab 17. The results showed that the polysaccharide content of Dendrobium candidum was the highest in the GA3 treatment group, and the polysaccharide content was up to 44.01% in the annial branch of Dendrobium candidum with GA3 treatment. Sunshading can increase the content of polysaccharides in the perennial branches of Dendrobium candidum. The content of polysaccharides in the 6-BA treatment group was significantly lower than that in the other treatment groups. Our study indicated that GA3 treatment can effectively improve the polysaccharide content of Dendrobium candidum, which is of great significance to strengthen the function of traditional Chinese medicine of Dendrobium candidum.