三叶青乙酸乙酯提取物对肝癌细胞HepG2凋亡的影响及机制

Effect of ethyl acetate extract of Tetrastigma hemsleyanum on apoptosis of HepG2 and its mechanism

目的:研究三叶青乙酸乙酯提取物对肝癌细胞HepG2增殖与凋亡的影响,并探究其作用机制。方法:HepG2细胞体外培养,在不同浓度三叶青提取物的作用下,采用CCK8法检测细胞增殖,应用流式细胞仪检测细胞凋亡和细胞周期,运用WesternBlot法检测蛋白表达情况。结果:三叶青乙酸乙酯提取物能显著抑制HepG2细胞增殖和诱导凋亡(P<0.05);上调Caspase-3和Bax表达量,下调Bcl-2表达,但细胞周期结果不显著。结论:三叶青乙酸乙酯提取物对HepG2细胞有抑制增殖作用,其机制可能与调控细胞信号通路诱导凋亡有关。

Objective: To study the effect of ethyl acetate extract of Tetrastigma hemsleyanum on the prolif-eration and apoptosis of HepG2 cells and to explore its mechanism. Methods: HepG2 cells were cul-tured in vitro. Under the action of different concentrations of extract, the cell proliferation was detect-ed by CCK8 method, cell apoptosis and cell cycle were detected by flow cytometry, Western blot was used to detect the protein expression.R esu lts: The ethyl acetate extract of Tetrastigma hemsleyanum could significantly inhibit the proliferation and induce apoptosis of HepG2 cells(P<0.05), and the ex-pression of Caspase-3 and Bax was up-regulated and the expression of Bcl-2 was down-regulated, but the cell cycle was not significantly affected by the ethyl acetate extract of Tetrastigma hemsleyanum.Conclusion: Tetrastigma hemsleyanum could inhibit the proliferation of HepG2 and its mechanism might be related to the regulation of apoptosis induced by cell signaling pathway.