基于质谱的磷酸化蛋白质组学:富集、检测、鉴定和定量

Phosphoproteomics Based on Mass Spectrometry(MS):Enrichment, Detection, Assignment and Quantification

磷酸化是一种调控生命活动的重要翻译后修饰,调控生物的生长发育、信号转导、以及疾病的发生发展.从上世纪80年代开始,质谱应用于蛋白质磷酸化的检测中,极大地推动了磷酸化蛋白质组学的发展.质谱检测拥有高灵敏度、高通量的特点,更重要的是具有位点分辨率,因此基于质谱的磷酸化蛋白质组检测方法得到不断的发展和推广.常见的磷酸化蛋白质组研究,首先对磷酸化肽段进行富集,然后进行串联质谱分析,最后通过搜索引擎对修饰位点进行鉴定和定量.本文从这个三个基本方面,对磷酸化蛋白质组研究进行综述,并对未来研究发展方向进行讨论.

Protein phosphorylation is one of post-translational modifications(PTM),which plays a role in regulation of development,signal transduction,and processes of diseases.Because of high sensitivity,considerable throughput,and residue resolution,mass spectrometry(MS)has been the most popular tool for phosphorylation modification analysis.Common MS-centric phosphoproteomics workflow includes phosphorylation modified peptides sampling,LC-MS/MS detection,phosphorylation sites assignment and quantification.We summarized and discussed these workflow parts in this review.