细辛不同药用部位马兜铃酸的含量测定

Determination of Aspartic Acid in Different Medicinal Parts of Asarum

通过高效液相色谱法,优化色谱条件,测定细辛不同批次、不同药用部位马兜铃酸的含量,为细辛药材的质量控制提供依据。方法采用高效液相色谱法,0.1%甲酸乙腈-水(40∶60)为流动相,C18色谱柱(4.6mm×150mm,5 m),流速1.0mL/min,检测波长320nm,柱温35℃,检测其限量成分马兜铃酸的含量。结果马兜铃酸在上述检测条件下检测限为0.4ng/mL,在0.001～0.200 g/mL范围内与峰面积有良好的线性关系(r=0.9985),平均加样回收率为98.09%,RSD为2.19%。检测结果显示不同批次细辛药材中地上部分均测出有痕量马兜铃酸,地下部分未能测出。结论该方法快速、准确,操作简单,专属性强,可为细辛药材的质量控制提供参考依据。

Objective To determine the content of aristolochic acid in different batches and different medicinal parts of Asarum by high performance liquid chromatography(HPLC) and provides basis for the quality control of Asarum medicinal materials and guarantee for later research. Methods The content of aristolochic acid was determined by 0.1% formic acid acetonitrile-water(40: 60)as the mobile phase, C18 column(4.6 mm×150 mm, 5μm), flow rate 1.0 mL/min, detection wavelength320 nm. column temperature 35 aC. Results The detection limit of aristolochic acid was 0.4 ng/mL under the above conditions,and there was a good linear relationship with the peak area in the range of 0.001~0.2μg/mL(r=0.9985). The average recovery rate was 98.09%. The value of RSD is was 2.19%. The test results showed that a trace amount of aristolochic acid was detected in the aerial parts of different batches of Asarum. and the underground part could not be measured. Conclusion The method is rapid, accurate. simple in operation and strong in specificity, which can provide reference for the quality control of Asarum medicinal materials.