不同采血部位和作用时间的微量元素检测结果差异分析

Difference analysis of microelement detection results between different blood sampling sites and action time

目的:探讨手指血和静脉血微量元素检测结果的差异程度和静脉血样本与试剂作用不同时间对微量元素检测结果的影响。方法:同时采集儿保中心100例体检患儿的手指末梢血和静脉全血。100例手指末梢血加入专用试剂混匀后放置30min为A组,100例静脉血加入专用试剂混匀后分别放置30min、60min、120min、180min、240min为B、C、D、E、F组;使用TC-3010D全自动微量元素分析仪对各组样本进行铅、铜、镉、锌、铁、钙、镁、锰8种元素的含量检测,并对结果进行比较分析(镉元素的检测结果分为<0.06μmol/L和>0.06μmol/L,无具体检测数值,因此本实验未对此元素结果进行统计分析)。结果:手指血和静脉血(A组和B组)的铜、锌、铁、钙、镁、锰元素检测结果差异无统计学意义(P>0.05),铅元素结果差异有统计学意义(P<0.05);静脉血与试剂不同作用时间的铅、铜、铁、锌、钙、镁、锰元素检测结果没有显著性变化(P>0.05),铁元素在作用60min和120min的检测结果比较差异无统计学意义(P<0.05),但与其他几个作用时间点检测结果相比差异有统计学意义(P<0.01)。相关性分析结果显示,作用时间与铁元素检测结果存在较强的正相关关系,(r=0.622,P<0.01);采血部位与铅元素检测结果存在弱正相关关系(r=0.166,P<0.05)。结论:最好采集静脉血进行微量元素检测,若使用手指血检测出现铅元素结果偏高时,必须抽取静脉血复检;建议血样在加入到专用试剂后,在1～2h内完成检测。

Objective:To investigate the difference of trace elements in finger blood and venous blood and Effects of different time of action of venous blood samples and reagents on microelement detection results.Method:At the same time,we collected 100 finger blood and venous blood from the child care center.100 cases of finger tip blood were mixed with special reagent and placed in group A for 30 minutes.100 cases of venous blood were mixed with special reagent and placed in group B,C,D,E,F for 30 minutes,60 minutes,120 minutes,180 minutes and 240 minutes respectively.TC-3010 Dautomatic microelement analyzer was used to detect the contents of lead,copper,cadmium,zinc,iron,calcium,magnesium and manganese in each group of samples,and the results were compared and analyzed(the results of cadmium detection were only<0.06μmol/L and>0.06μmol/L,and there was no specific detection value.The results of this experiment were not statistically analyzed).Result:There was no statistically significant difference in the detection results of copper,zinc,iron,calcium,magnesium and manganese in finger blood and venous blood(group A and group B)(P>0.05),and there was statistically significant difference in the results of lead(P<0.05).Venous blood and reagents in different time of lead,copper,iron,zinc,calcium,magnesium,manganese element test results did not change significantly(P>0.05),and iron in the role of60 min and 120 min there was no statistically significant difference compared to the test results(P<0.05),but compared with several other action time point test results have significant difference(P<0.01).The correlation analysis results showed that there was a strong positive correlation between the action time and the detection results of iron elements(r=0.622,P<0.01).There was a weak positive correlation between blood sampling site and lead detection results(r=0.166,P<0.05).Conclusion:It is best to collect venous blood for the detection of trace elements.If the lead content of finger blood test is high,it is necessary to extract venous blood for reexamination.It is suggested that the blood sample should be tested within 1 hto 2 hafter being added to the special reagent.