miR-335对牙囊干细胞成骨分化能力的影响

The effects of miR-335 on the osteogenic differentiation potential of the DFSCs

目的体外研究大鼠牙囊干细胞成骨分化能力,以及miR-335对牙囊干细胞成骨分化能力的影响。方法双向差速法体外分离培养大鼠牙囊干细胞,进行成骨诱导。用茜素红染色检测成骨分化的能力,进而用qRT-PCR检测miR-335的表达,并且在瞬时转染miR-335 mimics和inhibitor后成骨诱导,用PCR和Western Blot的方法检测成骨能力的改变。结果双向差速法培养的牙囊干细胞具有成骨样细胞分化能力。成骨诱导的牙囊干细胞中miR-335表达降低,转染mimics后成骨能力降低,转染inhibitor后成骨能力升高。结论在体外条件miR-335可以抑制牙囊干细胞成骨分化。

Objective To study the osteogenic differentiation potential of dental follicle stem cell (DFSCs) in virto and the effects of miR335 on the osteogenic differentiation potential of DFSCs. Methods DFSCs were purified through in vitro culture combined with bidirectional differential method. After osteogenesis induction, the osteogenic differentiation potential was determined by alizarin red staining. miR335 expression was analyzed by qRTPCR. Western blot and PCR were used to evaluate the osteogenic differentiation potential of DFSCs after transient transinfection of miR335 mimics or inhibitor. Results DFSCs cultured by bidirectional differential method had osteogenesis ability. miR335 expression in DFSCs was lower than that in osteogenic induced DFSCs. The osteogenic differentiation potential of DFSCs was reduced by miR335 mimics, while promoted by miR335 inhibitor. Conclusion miR335 in virto may have a negative effect on the ostegenic differentiation of DFSCs.