超高效液相色谱-质谱检测尿液中马尿酸和甲基马尿酸

Determination of hippuric acid and methyl hippuric acid in urine by ultra high performance liquid chromatography-mass spectrometry

目的建立超高效液相色谱-质谱(UPLC-MS/MS)检测尿液中马尿酸和甲基马尿酸的新方法。方法实验采用反相T3色谱柱,以乙腈-甲醇-0. 1%甲酸水溶液为流动相,等度洗脱,通过电喷雾三重四级杆质谱检测。结果该方法实现了马尿酸和甲基马尿酸的三个同分异构体的基线分离和检测,方法检出限低(马尿酸0. 07μg/L,甲基马尿酸0. 1μg/L),线性范围广(1～10 000μg/L均具有良好的线性关系)。结论超高效液相色谱-质谱检测尿液中马尿酸和甲基马尿酸具有较好的应用前景。

Objective To develop a novel method for the determination of hippuric acid and methyl hippuric acid in urine by ultra high performance liquid chromatography-mass spectrometry (UPLC-MS/MS). Methods We employed reversed phase T3 column and acetonitrile-methanol-water (with 0.1% formic acid) as elution solvent, and then detected hippuric acid and methyl hippuric acid in urine by electrospray ionization mass spectrometry. Results This method successfully realized the baseline separation and detection of hippuric acid and three isomers of methyl hippuric acid, and obtained low detection limits (0.07μg/L for hippuric acid and 0.1μg/L for methyl hippuric acid) and a wide liner range (1-10,000 μg/L). Conclusion Ultra high performance liquid chromatography-mass spectrometry has a promising application prospect in the determination of hippruic acid and methyl hippuric in urine.