超声波辅助提取伊犁薰衣草花色苷工艺及其抗氧化活性研究

Ultrasonic-assisted extraction and antioxidant activity of anthocyanins in lavender from Xinjiang

以新疆伊犁薰衣草为研究对象,研究了超声波辅助提取花色苷的工艺条件。以薰衣草花色苷得率为响应值,在单因素实验的基础上,通过Box-Behnken设计实验结合响应面分析法确定了超声辅助提取伊犁薰衣草花色苷的最佳实验条件为:温度为63℃、提取剂为61%的乙醇、料液比为38:1 mL/g、超声提取27 min,该条件下花色苷得率为4.88%。通过测定薰衣草花色苷的还原能力以及清除DPPH·自由基、超氧阴离子自由基(O_2~—·)、羟基自由基(·OH)和亚硝酸根离子的能力来评价其抗氧化活性,结果表明薰衣草花色苷具有一定的体外抗氧化能力。

In this research,the optimum conditions for the ultrasonic-assisted extraction of anthocyanins from the flowers of lavender from Xinjiang were studied.The influences of some factors on the yield of lavender anthocyanins were investigated.On the basis of single-factor experiments,the optimum extracting conditions were determined by the method of Box-Behnken design coupled with response surface methodology.The results were as the following:63℃ of extraction temperature,61% of ethanol concentration,27 min of extraction time,rate of solution to solid 38:1mL/g.Under these conditions,the actual average value was 4.88%.The antioxidant activity of lavender anthocyanins was evaluated by scavenging DPPH.,O2^-·,·OH and nitrite ions.The results indicated that lavender anthocyanins had a certain antioxidant activity in vitro.