消炎抗菌片的抗菌活性及对福氏志贺氏菌的抗菌机制初步探讨

Antibacterial activity of Xiaoyankangjun Tablets and preliminary discussion of antibacterial mechanism against Shigella flexneri

目的:研究武汉大学人民医院院内制剂消炎抗菌片对14种不同菌株的抑菌活性,并初步探讨其对福氏志贺菌(Shigella flexner)的抑菌机制。方法:采用K-B法和液体二倍稀释法,测定消炎抗菌片对14种常见致病菌株的抑菌活性,并以药物敏感性较强且与药物主要临床疗效相关的福氏志贺菌为受试菌,从药物对该细菌生长特性的影响、电导率变化,以及SDS聚丙烯酰胺凝胶电泳(SDS-PAGE)谱带,考察给药后菌体变化情况。结果:消炎抗菌片对福氏志贺菌、铜绿假单胞杆菌、藤黄微球菌、大肠杆菌、金黄色葡萄球菌等14个菌种及菌株具有不同程度的抑制作用,总体对革兰阴性菌抑菌效果比革兰阳性菌强;药物作用福氏志贺菌后,菌悬液电导率与对照组相比显著升高,SDS-PAGE结果显示经消炎抗菌片处理的菌体无明显蛋白条带形成。结论:本院院内制剂消炎抗菌片对14种菌株具有不同程度的抗菌活性,能够显著影响福氏志贺菌的细胞膜通透性,且对菌体蛋白具有一定抑制作用。

OBJECTIVE To study the antibacterial activities of Xiaoyankangjun tablets in our hospital on 14 different strains, and to explore its antibacterial mechanism against Shigella flexner. METHODS Using K-B method and liquid double dilution method, the antibacterial activities of Xiaoyankangjun tablets against 14 common pathogenic strains was determined. Shigella flexneri, which had strong drug sensitivity and was related to the main clinical efficacy of the drug, was used as the test bacteria. The effects of the drug on the growth characteristics of the bacteria, the change of conductivity, and the SDS-PAGE by SDS-PAGE were used to investigate the changes of the cells after medication.RESULTS Xiaoyankangjun tablets have different degrees of inhibition on Shigella flexneri, Pseudomonas aeruginosa, Micrococcus luteus, Escherichia coli, Staphylococcus aureus and other strains, and overall inhibition against gram negative bacteria. The effect was generally stronger to gram negative bacteria than to gram positive bacteria. After Shigella flexneri was treated by the drug, the conductivity of the bacterial suspension was significantly higher than that of the control group. The SDS-PAGE results showed that the cells treated with the anti-inflammatory antibacterial tablets had no obvious protein strips.CONCLUSION The Xiaoyankangjun tablets in our hospital have different degrees of antibacterial activity against 14 strains, and significantly affect the cell membrane permeability of Shigella flexneri and have a certain inhibitory effect on bacterial proteins.