不同来源的蛹虫草子实体活性成分的比较

Comparison of the active components in fruit bodies of different strains of Cordyceps militaris

通过比较不同来源的蛹虫草子实体的活性成分以探讨蛹虫草品质的差异。对17个蛹虫草菌株栽培得到的子实体及16个市售样品中的多糖、核苷类成分、游离糖醇及小分子糖类的含量进行分析测定,并比较其核苷类成分HPLC指纹图谱。结果表明,因菌株不同蛹虫草子实体的活性成分具有不同程度的差异,菌株对虫草素含量的影响最大,其次是N6-(2-羟乙基)腺苷,因菌株不同虫草素含量可相差14倍,N6-(2-羟乙基)腺苷的含量差异可达6倍以上。市售样品中的核苷类成分分析结果也证明了在测定的几种成分中,虫草素是含量差异最大的活性成分。17个蛹虫草菌株子实体的多糖含量为1.81%–4.92%,甘露醇含量为1.44%–4.47%,海藻糖含量为3.58%–25.43%。16个市售样品的多糖含量为2.84%–5.55%,甘露醇含量为0.96%–3.93%,海藻糖含量为1.04%–19.91%。采用数据归一化法进行子实体品质综合评价研究,结果表明菌株G7a、G10a、G15a综合品质较好,多数成分含量均大于平均值,是生产高品质蛹虫草的合适菌株。

Comparison of the quality of different Cordyceps militaris fruit body samples was made,based on comparing the active compositions.The content of polysaccharide,nucleosides,free alditol and low molecular weight sugar in the fruit bodies from 17 different C.militaris strains and 16 samples on market were determined and their HPLC spectrum of nucleosides were compared.Results indicated that the active components in C.militaris fruit bodies varied with different strains.The strain quality had great influence on the content of cordycepin and N6 -(2-hydroxyethyl)adenosine.The difference between the lowest and the highest content of cordycepin in the samples reached fourteen fold,while that of N6 -(2-hydroxyethyl)adenosine more than six fold.The nucleoside analysis of C.militaris samples on the market also indicated the great variation of cordycepin content.The content of polysaccharide,mannitol and trehalose in fruit bodies of 17 strains was 1.81%-4.92%,1.44%-4.47% and 3.58%-25.43% respectively,while that in 16 samples on the market was 2.84%-8.55%,0.96%-3.93% and 1.04%-19.91%,respectively.Fruit body quality was comprehensively evaluated based on data normalization method.Fruit bodies of strains G7a,G10a and G15a had good comprehensive quality with most of the components beyond the average values,indicating these strains were suitable for producing high quality C.militaris fruit bodies.