不同前处理方法对生鲜乳中布鲁氏杆菌DNA检测结果的影响

Effect of different pretreatment methods on the detection of Brucella DNA in fresh and raw milk

为了研究不同前处理方法对生鲜乳中布鲁氏杆菌DNA检测结果的影响,试验采用PBS冲洗法、4℃过夜法、离心法三种方法对乳样进行前处理,用多功能酶标仪和琼脂糖凝胶电泳法对提取的DNA质量进行评估。结果表明:采用4℃过液法得到的OD260/OD280值最高且得到的条带最亮,采用PBS法得到的DNA浓度最高。因此,采用4℃过夜法提取乳样中布鲁氏杆菌基因组DNA的效果最好。

The aim of the present study was to investigate the effect of different pretreatment methods on Brucella DNA in fresh milk. The milk samples were pretreated by PBS rinsing, 4 ℃ overnight and centrifugal method. Then the quality of the extracted DNA was evaluated by multifunctional microtiter and agarose gel electrophoresis. The results showed that the 4 ℃ overnight method was the best method to extract genomic DNA of Brucella.