CD33 rs3865444多态性与红水河流域长寿人群血脂水平的相关性

Association study between CD33 rs3865444 polymorphism and lipid levels in the long-lived inhabitants residing in Guangxi Hongshuihe River Basin

目的 探讨广西红水河流域长寿人群CD33 rs3865444多态性与血脂水平的相关性。方法 应用改良的多重高温连接酶检测反应技术(iMLDR)对该流域壮族长寿老人(长寿组,≥90岁,n=645)、非长寿老人(当地对照组,60~77岁,n=674)以及贺州非长寿家系(外地对照组,60~77岁,n=662)进行CD33 rs3865444多态性位点进行基因分型,并分析各基因型对体重指数、血压、空腹血糖及血脂指标等心血管危险因素的影响。结果 红水河流域女性CD33 rs3865444等位基因A频率及基因型(CA/AA)频率明显高于外地对照组,长寿组女性CA/AA基因型频率明显高于男性,而外地对照组女性CA/AA基因型频率则明显低于男性。长寿组男性A等位基因携带者TC、LDLC及载脂蛋白B水平明显高于非A等位基因携带者(P均<0.0001);当地对照组女性A等位基因携带者HDLC低于非A等位基因携带者,而外地对照组女性A等位基因携带者HDLC及载脂蛋白A则高于非A等位基因携带者。结论 CD33 rs3865444多态性与血脂水平有一定的关系,但受性别及环境等因素的影响。A等位基因在长寿女性中富集的意义有待进一步研究。

Aim To investigate the possible relationship between variant CD33 rs3865444 and serum lipid levels in long-lived population inhabiting along Hongshuihe River Basin in Guangxi province.Methods Genotyping of CD33 rs3865444 locus was performed by improved multi-temperature ligase detection reaction technique (iMLDR) for long-lived individuals (long-lived group, aged≥90 years, n=645), non-long-lived elderly (local control group, aged 60~77 years, n=674) residing in Hongshuihe River Basin and non-long-lived elderly living in Hezhou (non-local control group, aged 60~77 years, n=662), a town about 600 km from Guangxi Honeshuihe.Association of CD33 rs3865444 genotypes with lipids was analyzed thereafter.Results The frequencies of A allele and its genotype (CA/AA) of females in Hongshuihe River Basin, both long-lived group and long control group, were significantly higher than those of non-local controls; CA/AA genotype of females was higher than that of males in the long-lived group, while this status was opposite between gender in the non-local controls.The levels of TC, LDLC and ApoB of A allele carriers in males in long-lived group were dramatically greater than those of non-A allele carriers (P<0.0001), while HDLC level of A carriers was lower than non-A carriers in females of local controls; by contrary, HDLC and ApoA levels of females were higher than those of non-A carriers in the non-local controls.Conclusions CD33 rs3865444 polymorphism correlates with lipid levels to some extent, which is influenced by sex and other environmental factors.The enrichment of A allele of CD33 rs3865444 locus in the long-lived females needs further clarification.