欧亚禽系H1N1猪流感病毒在中国的流行和遗传进化

Prevalence and genetic evolution of EA-H1N1 swine influenza virus in China

H1N1猪流感病毒(swine influenza virus, SIV)对公共卫生构成了潜在的威胁,流感病毒基因组序列的遗传进化及特定的氨基酸差异可以影响病毒的致病性和毒力.本研究组对中国猪流感的流行情况进行统计分析,发现中国主要流行的是欧亚禽系H1N1亚型SIV,进一步对欧亚禽系的H1N1 SIVs进行基因型分析发现其可分为11个基因型,并且重配型的欧亚禽系H1N1亚型SIV,特别是病毒的PB2, PB1, PA和NP基因源于pandemic H1N1/2009的重配毒株近年来出现得越来越频繁.此外,从湖北省分离到的1株新的欧亚禽系猪流感病毒(A/swine/Hubei/221/2006(H1N1)),通过与中国欧亚禽系H1N1猪流感病毒的同源比较发现,该分离株的基因组片段与此前从人体内分离到的欧亚禽系猪流感病毒A/Hunan/42443/2015(H1N1)的基因组片段亲缘关系很近.总之,本研究通过对中国欧亚禽系H1N1猪流感病毒的流行状况和遗传进化进行分析,为猪流感的预防和控制提供了一定的理论依据.

H1N1 swine influenza virus (SIV)has posed a potential threat to public health.Genetic evolution and amino acid substitutions of whole genome sequence of influenza virus have been shown to be associated with the virulence and pathogenicity of the virus.We made a statistical analysis of the prevalence of swine influenza virus in China and found that Eurasian avian-like H1N1(EA-H1N1) SIVs were the main prevalent SIVs in China.We further analyzed the genotypes of EA-H1N1 SIVs,and the results showed that these viruses were divided into 11 genotypes,and there is an increasing number of reassorted EA-H1N1 SIVs in recent years,especially for those viruses whose viral genes PB2,PBI,PA and NP derived from pandemic H1N1/2009.Besides,we isolated a novel EA-H1N1 SIV (A/swine/Hubei/221/2006(H1N1)).We find that this isolated virus is highly homologous to a previous human isolated EA-H1N1 virus A/Hunan/42443/2015(H1N1).Taken together,this study analyzes the prevalence and genetic evolution of EA-H1N1 SIVs in China,which provides a theoretical basis for the prevention and control of swine flu.