摘要
目的了解上海市动物园蛇曼氏裂头蚴感染情况。方法 2017年对上海市动物园送检的30条蛇进行解剖检查,分离曼氏裂头蚴,记录寄生部位和数量;新鲜虫体经盐酸卡红染色后,于显微镜下进行形态学鉴定。提取虫体基因组DNA, PCR扩增曼氏裂头蚴线粒体基因细胞色素c氧化酶亚单位1 (cytochrome c oxidase subunit 1, cox1)、细胞色素b (cytochrome b, cytb),并进行测序分析。结果上海市动物园送检蛇的裂头蚴总感染率为86.7%(26/30),共检获裂头蚴626条,感染度为24.1条/蛇。其中6条王锦蛇中有3条感染裂头蚴,感染度为1.33条/蛇;乌梢蛇的感染率为95.8%(23/24),感染度为27.0条/蛇。裂头蚴主要寄生于蛇肌肉组织,占总数的52.9%(331/626)。显微镜下观察,所获虫体具有曼氏裂头蚴的形态特征。cox1基因和cytb基因的PCR扩增产物分别约为470 bp和1 400 bp。对26条蛇中分离的裂头蚴cox1和cytb基因序列一致性分析结果显示,cox1基因的序列一致性为96.4%~100%, cytb基因的为97.8%~100%。与GenBank中的绦虫序列进行比对,发现其与欧猥迭宫绦虫(GenBank登录号为KP738288、 AB374543)的序列一致性为99.8%~100%,与其他种类绦虫的序列一致性为54.2%~84.4%。结论上海市动物园送检蛇的曼氏裂头蚴感染率较高,蛇的曼氏裂头蚴可感染人类,需加强防控。
Objective To investigate the infection status of plerocercoid in snakes from Shanghai Zoo in order to provide information for the prevention and treatment of sparganosis. Methods Thirty snakes were provided from Shanghai Zoo and dissected in 2017. The plerocercoids were collected from the snakes, the number of plerocercoids and their location were recorded. The structure of plerocercoid was examined under microscope after being Carmine stained. The total DNA was extracted from the plerocercoids and the DNA fragments for cytochrome c oxidase subunit 1 (cox1) and cytochrome b (cytb) genes were amplified by PCR. The amplified PCR products were sequenced and analyzed. Results The infection rate of plerocercoid in the snakes was 86.7% (26/30) with total 626 plerocercoids found, with an average intensity of infection of 24.1 plerocercoids per snake. The infection rate in snake Elaphe carinata and Zaocys dhumnades was 50.0% (3/6) and 95.8% (23/24), with the average intensity of infection of 1.3 and 27.0 plerocercoids per snake, respectively. Muscular tissue was the most common parasitizing location for the plerocercoids, accounting for 52.9% (331/626). The isolated worms were in accordance with Spirometra mansoni plerocercoids in morphology. The PCR amplified product of cox1 gene was about 470 bp and cytb gene was about 1 400 bp, respectively, sharing 96.4%~100% (cox1) and 97.8%~100% (cytb) sequence identity among the 26 samples. In comparison with the different tapeworm sequences in GenBank, it was found that the amplified cox1 and cytb gene sequences in the plerocercoids were highly homologous to those of S. erinaceieuropaei (99.8%~100%, GenBank Accession No. KP738288, AB374543) and less homologous to other species of tapeworm. Conclusion The snakes collected from Shanghai Zoo are widely infected with S. mansoni plerocercoids, indicating that they can be the infection source of human sparganosis.
作者
卢艳
陈家旭
李浩
蔡玉春
艾琳
储言红
宋鹏
陈韶红
LU Yan;CHEN Jia-xu;LI Hao;CAI Yu-chun;AI Lin;CHU Yan-hong;SONG Peng;CHEN Shao-hong(National Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention,Chinese Center for Tropical Diseases Research)
出处
《中国寄生虫学与寄生虫病杂志》
CAS
CSCD
北大核心
2018年第6期593-596,共4页
Chinese Journal of Parasitology and Parasitic Diseases
基金
上海市卫计委科研课题面上项目(N0.201740076)
国家寄生虫种质资源共享服务平台(N0.TDRC-22)
国家重点研发计划项目(N0.2016YFC1202000,N0.2016YFC1202005).
关键词
曼氏裂头蚴
蛇类
分子鉴定
动物园
Spirometra mansoni plerocercoid
Snake
Molecular identification
Zoo