细胞因子IL-26在生殖道炎患者体内表达及意义

Expression and Significance of Cytokine IL-26 in Patients with Genital Tract Inflammation

目的:通过检测生殖道炎患者血清及生殖道分泌液中多种细胞因子的表达水平,研究其在生殖道炎中的作用及可能的分子机制。方法:收集临床147例生殖道炎患者和90例健康人的血清及生殖道分泌液样品,分别采用ELISA法测定IL-26、IL-17和IL-37的表达水平,分析IL-26与IL-17及IL-37之间的相关性。分离急性期生殖道炎患者外周血CD4+T细胞和单核细胞并共培养,IL-26、抗-CD3抗体刺激后,检测培养上清Th17型细胞因子IL-17、IL-23和Treg细胞因子IL-10、TGF-β1的表达。结果:生殖道炎患者血清及生殖道分泌液中IL-26表达水平明显上调(P<0.001),且与IL-17的表达呈正相关(r=0.915、0.673),与IL-37的表达呈负相关(r=0.78、0.77)。IL-26能促进CD4+T细胞和单核细胞的IL-17和IL-23细胞因子的分泌,抑制IL-10和TGF-β1细胞因子的产生。结论:生殖道炎患者体内IL-26表达上调,并且促进Th17型细胞因子分泌和抑制Treg细胞相关因子的分泌,因此IL-26有潜力成为治疗生殖道炎的新靶点。

Objective To study the role and possible molecular mechanism of cytokines in genital tract inflammation by detecting the expression levels of cytokines in serum and secretion of genital tract in patients with genital tract inflammation. Methods Serum and genital secretion samples from 147 patients with genital tract inflammation and 90 healthy persons were collected.The expression levels of IL-26,IL-17 and IL-37 were determined by ELISA,and the correlation between IL-26,IL-17 and IL-37 was analyzed.CD4+T cells and monocytes from peripheral blood of patients with acute genital tract inflammation were isolated and co-cultured.After stimulation by IL-26and anti-CD3 antibodies,the expression of Th17 cytokines IL-17,IL-23,Treg cytokines IL-10 and TGF-beta 1 in culture supernatants were detected.Results The expression of IL-26 in serum .and genital tract fluid of patients with chronic genital tract inflammatory disease was siguifieantly up-regulated(P<0.001).The expression of IL-26 was positively correlated with the expression of IL-17(r=0.915,0.673) and negatively correlated with the expression of IL-37(r=0.78,0.77).IL-26 could promote the secretion of IL-17 and IL-23 and inhibit the production of IL-10 and TGF-β1 in CD4+T cells and monocytes.Conclusion IE-26 expression is up-regulated in patients.with genital tract inflammation,promotes the secretion of Th17-type cytokines and inhibits the secretion of Treg-associated factors.Therefore,IL-26 has the potential to become a new target for the treatment of genital tract inflammation.