慢性鼻-鼻窦炎细菌生物膜与Th17/Treg失衡的研究

Involvement of Th17/Treg imbalance in bacterial biofilm induced chronic rhinosinusitis

目的:探讨Th17/Treg失衡在细菌生物膜(BBF)参与的慢性鼻-鼻窦炎(CRS)发病中的作用。方法:收集CRS鼻窦黏膜,以激光共聚焦显微镜(CSLM)检测BBF,选取BBF阳性和阴性者各20例,正常鼻腔鼻窦黏膜10例,Real-time PCR法检测叉状头转录因子3(Foxp3)、维甲酸相关孤核受体γt(RORγt)、IL-17的mRNA表达,分别比较CRS组与对照组、BBF阳性组和BBF阴性组的差异。相关性分析研究基因表达之间的关系。结果:CRS组与对照组相比Foxp3mRNA降低(P<0.05),提示Treg细胞功能减弱;两组之间的IL-17和RORγt的mRNA水平无差异(P>0.05)。BBF阳性组与BBF阴性组相比,Foxp3mRNA降低,IL-17mRNA升高(均P<0.05),提示Th17细胞功能增强,Treg细胞功能减弱;两组之间RORγt mRNA水平无差异(P>0.05)。Foxp3与IL-17mRNA水平呈负相关(r=-0.283,P<0.05);Foxp3和RORγt、IL-17和RORγt的mRNA水平无显著相关性(P>0.05)。结论:BBF引起CRS鼻窦黏膜Th17/Treg失衡,可能是CRS的重要致病因素。

Objective:The aim of this study is to investigate the involvement of Th17/Treg imbalance in the pathogenesis of bacterial biofilm(BBF)induced chronic rhinosinusitis(CRS).Method:CRS nasal sinus mucosa was collected,and BBF was examined by BacLight/CSLM detection.Forty CRS cases(20 BBF positive cases and20 negative cases)and 10 controls were enrolled.Clinical data were recorded before operation,and nasal sinus mucosa was collected during operation.The mRNA level of Forkhead box protein 3(Foxp3),retinoid-related orphan nuclear receptorγt(RORγt),and IL-17 were detected by Real-time PCR.The differences between CRS and controls,as well as BBF positive and BBF negative group were analyzed.Correlation analysis was conducted to study the relationship between gene expression.Result:The Foxp3 mRNA was decreased in the CRS group compared with the control group(P<0.05),suggesting that the function of Treg cells was weakened.there was no difference in the mRNA level of IL-17 and RORγt between the two groups(P>0.05).Compared with the BBF-negative group,the Foxp3 mRNA was decreased and the IL-17 mRNA was increased in the BBF-positive group(P<0.05),suggesting that Th17 cells function enhanced and Treg cells function decreased.The mRNA level of RORγt showed no difference(P >0.05).The mRNA level of Foxp3 and IL-17 was negatively correlated(r=-0.283,P<0.05).Conclusion:The imbalance of Th17/Treg in sinus mucosa caused by BBF may play an important role in CRS.