化橘红多糖对环磷酰胺所致免疫损伤小鼠脾脏抗氧化能力的影响

Effect of Citri Grandis Exocarpium polysaccharide on antioxidant capacity of spleen in mice with immunological injury induced by cyclophosphamide

本文选用岭南地区的特色贵重药材化橘红作为实验材料,采用回流提取,95%醇沉淀的方法提取除了化橘红粗多糖组分,考察谷胱甘肽过氧化物酶(GSH-PX)、丙二醛(MDA)及超氧化物歧化酶(SOD)在由腹腔注射环磷酰胺所导致免疫损伤型小鼠脾脏的组织匀浆中累积情况的变化。6组小鼠在连续给药15d后采用摘除眼球采血,脱颈处死后,试剂盒检测小鼠脾脏组织匀浆中SOD、MDA及GSH-PX指标的变化情况。结果表明,给予化橘红多糖可显著提高小鼠脾脏中SOD和GSH的活力,降低小鼠血清中MDA的含量,这就是化橘红多糖对环磷酰胺所致小鼠免疫损伤产生抗氧化保护作用的原因所在。

In this paper,the components of Citri Grandis Exocarpium polysaccharides were extracted by water extraction and alcohol precipitation,and their effects on SOD,MDA and GSH-PX in the spleen of immunosuppressed mice induced by cyclophosphamide were investigated.48 mice were randomly divided into 6 groups:blank control group,model group(cyclophosphamide),positive drug group(levamisole hydrochloride),high dose group,medium dose group and low-dose group,except for the blank control group,the other groups were intraperitoneally injected with cyclophosphamide for three consecutive days to establish an immunosuppressive model.After 10 days of continuous intragastric administration,the mice in each group were removed from the eye.The SOD,MDA and GSH-PX indicators in the spleen of mice were detected.The results showed that the Citri Grandis Exocarpium polysaccharide could significantly increase the activity of SOD and GSH in the spleen of mice,and reduce the content of MDA in the serum of mice.This is the protective mechanism of the Citri Grandis Exocarpium polysaccharide on the immune damage induced by cyclophosphamide in mice.