蒙药芯芭中6个环烯醚萜苷的HPLC定量分析

HPLC Analysis of Six Iridoid Glycosides from Cymbaria dahurica L.

目的建立芯芭中6个环烯醚萜苷的含量测定方法。方法采用高效液相色谱法对不同来源芯芭进行含量测定,色谱条件:色谱柱为Inertsil ODS-SP column(4. 6 mm×250 mm,5μm),以乙腈-水为流动相梯度洗脱,检测波长为210 nm,柱温为30℃。结果在选用实验色谱条件下,化合物(S1~S6)的校准曲线表现出良好的线性关系(r> 0. 999 4),平均回收率在95. 0%~98. 0%内,RSD均小于3. 0%。不同来源样品中S1、S2、S3、S4、S5和S6的含量随产地的不同而有显著差异。结论通过实验研究,建立了芯芭中6个环烯醚萜苷的含量测定方法,为综合评价该药材质量提供依据。

OBJECTIVE To develop a method for simultaneous determination of six iridoid glycosides from Cymbaria dahurica.METHODS Cymbaria dahurica collected from different regions were determined by HPLC. The separation was performed on Inertsil ODS-SP column( 4. 6 mm × 250 mm,5 μm) at 30 ℃ by gradient elution with CH3 OH-H2 O as the mobile phase,with the detection wavelength set at 210 nm. RESULTS Under the chromatographic conditions adopted in this study,all the calibration curves exhibited good linearity( r > 0. 999 4) in a relatively wide concentration range. The recovery of the method was within the range of 95. 0%-98. 0%,and the RSD was less than 3%. The contents of all the compounds( S1-S6) in samples from different habitats varied significantly. CONCLUSION The quantitative method for evaluating the quality of Cymbaria dahurica is established and can be used for the quality control of Cymbaria dahurica.