川贝母-枇杷叶配伍对枇杷叶中3种黄酮成分血浆药动学的影响

Effect of Drug Pair of Fritillaria Cirrhosa-Eriobotryae Folium on Plasma Pharmacokinetics of Three Flavonoids from Eriobotryae Folium

目的通过高效液相色谱-串联质谱法(LC-MS/MS)方法,探讨川贝与蜜炙枇杷叶配伍后,枇杷叶中芦丁、槲皮素和山柰酚的药动学变化。方法血浆样品经固相萃取处理。色谱柱为Waters XTerra MS C_(18)柱(150 mm×2.1 mm,3.5μm);流动相为乙腈-0.1%甲酸溶液;质谱采用电喷雾离子源,负离子模式(ESI^-),多反应监测模式(MRM)进行定量。大鼠随机分2组,分别灌胃单味枇杷叶和药对川贝-枇杷叶水煎液,测定药动学参数。结果待测物线性关系良好(r^2≥0.998),精密度、准确度、基质效应和提取回收率满足定量要求。与单药组相比,药对组中芦丁、槲皮素和山柰酚的C_(max)和AUC_(0-t)均下降,t_(1/2)推迟。结论川贝与枇杷叶配伍后,降低了芦丁、槲皮素和山柰酚的最大血药浓度,减慢了消除率,延长了它们在体内的驻留时间。

OBJECTIVE To investigate the effects of the compatibility of Eriobotryae Folium and honey-fried Fritillaria Cirrhosa on pharmacokinetic changes of rutin,quercetin,kaempferol by LC-MS/MS method.METHODS The plasma samples were extracted by solid-phase extraction.The LC column was Waters XTerra MS C 18 (150 mm×2.1 mm,3.5 μm).The mobile phase comprised of acetonitrile and water containing 0.1% formic acid.The quantification was obtained by scanning with multiple reaction monitoring (MRM) via the negative electrospray ionization (ESI - ) source.The rats were randomly divided into two groups and orally administered with Eriobotryae Folium or Eriobotryae Folium-Fritillaria Cirrhosa decoction.RESULTS The correlation coefficients were over 0.998,indicating excellent linearity.The values of precision,accuracy,matrix effect and average recovery were acceptable.Compared with single herb group,the C max and AUC 0-t were decreased,the t 1/2 were extended in herbal pair group.CONCLUSION After combining the herbs,the bioavailability of rutin,quercetin and kaempferol are reduced,the elimination of them delays,and the dwell time increases.