摘要
目的:探究内皮祖细胞(endothelial progenitor cell,EPCs)的原代培养及鉴定方法,并探讨益气活血组方对大鼠骨髓源性内皮祖细胞增殖、迁移和黏附的影响。方法:将EPCs随机分为空白血清组、5%、10%、15%含药血清组和对照组,以相应血清干预原代EPCs,并分别通过CCK8法、Transwell迁移实验、黏附实验评估对EPCs的增殖、迁移、黏附的影响。结果:空白血清组和含药血清组EPCs增殖、迁移、黏附能力均高于对照组(P<0.05或P<0.01)。10%、15%含药血清组EPCs增殖、迁移、黏附能力均高于空白血清组(P<0.05);10%、15%、5%含药血清组与空白血清组比较,差异均无统计学意义(P>0.05)。结论:益气活血组方能提高大鼠骨髓源性内皮祖细胞增殖、迁移和黏附能力,且与含药血清浓度值呈正相关,并且血清浓度的平台值为15%,超过这个阀值时,血清浓度的增高对细胞的生长影响不大。
Objective:To explore the primary culture and identification methods of EPCs,and to observe the effect of Yiqi Huoxue Prescription (益气活血方,YQHXP)on rat bone marrow derived endothelial progenitor cell proliferation,migration and adhesion.Methods:EPCs were randomly divided into blank serum group,5%, 10% and 15% drug-containing serum group and control group.The primary EPCs were intervened with corresponding serum.The effects on EPCs proliferation,migration and adhesion were evaluated by CCK8 method, Transwell migration test and adhesion test,respectively.Results:The proliferation,migration and adhesion of EPCs in blank serum group and drug-containing serum group were higher than those in control group (P<0.05 or P<0.01).EPCs proliferation,migration and adhesion ability in 10% and 15% drug-containing serum group were higher than those in blank serum group (P<0.05);there was no significant difference between 10% drugcontaining serum group and 15% drug-containing serum group and 5% drug-containing serum group and blank serum group (P>0.05).Conclusion:Yiqi Huoxue prescription can improve the proliferation,migration and adhesion ability of rat bone marrow-derived endothelial progenitor cells,which is positively correlated with the concentration of drug-containing serum,and the plateau value of serum concentration is 15%.When the plateau value exceeds this threshold,the increase of serum concentration has little effect on cell growth.
作者
乐金海
王瑾茜
胡国恒
LE Jin-hai;WANG Jin-xi;HU Guo-heng(The First Affiliated Hospital of Hu'nan University of Chinese Medicine,Changsha Hu'nan 410007,China)
出处
《中医药导报》
2018年第24期21-25,共5页
Guiding Journal of Traditional Chinese Medicine and Pharmacy
基金
湖南省科技计划项目(2015SK2016
2015SK20161)
关键词
益气活血组方
大鼠骨髓源性内皮祖细胞
增殖
迁移
黏附
Yiqi Huoxue prescription
rat bone marrow derived endothelial progenitor cells
proliferation
migration
adhesion