不同强度运动训练对青春期大鼠学习记忆能力的影响及其机制研究

Effect of different intensities of exercise training on learning and memory in adolescent rats and the underlying mechanism

目的观察不同强度的运动训练对青春期大鼠学习记忆能力的影响以及和血清瘦素水平的关系,为研究青春期学习记忆能力的提高提供依据。方法选择30只45~50日龄大鼠,随机分为低强度运动组、中等强度运动组和对照组,每组10只,连续一周低强度或中等强度跑台运动训练,Morris水迷宫观察大鼠学习记忆,酶联免疫法检测血清瘦素(Lep)水平。结果中等强度运动训练组大鼠Morris水迷宫实验四个象限的平均逃避潜伏期分别为(44.8±5.0)s,(49.3±5.2)s,(39.3±4.7)s和(37.7±2.8)s,均短于正常组大鼠(P<0.01),中等强度运动训练组大鼠穿越站台次数为5.2±1.8,高于正常组大鼠(2.6±0.4),差异均具有统计学意义(P<0.01)。中等强度运动组大鼠的血清Lep水平[(15.1±4.5)pg/ml]高于正常组大鼠[(12.8±3.7)pg/ml](t=2.47,P<0.05)。结论中等强度的运动训练能够提高青春期大鼠的学习记忆能力,这可能与血清Lep含量的增加有关。

Objective To observe the effect of different intensities of exercise training on learning and memory of adolescent rats,as well as the relationship between training and serum leptin levels. Methods Totally 30rats aged 45-50days were selected and randomly divided into three groups,including low intensity exercise,moderate intensity exercise and control group,with 10rats in each group.Morris water maze was used to observe the learning and memory of rats.The serum leptin level was tested by enzyme-linked immunosorbent assay(ELISA). Results The average escape latency of four morris water maze tests in the moderate intensity exercise was(44.8±5.0)s,(49.3±5.2)s,(39.3±4.7)s and(37.7±2.8)s,respe ctively,which were all shorter than those of control group(P<0.01).The number of crossing platform and the level of serum leptin in the moderate intensity exercise training group were significantly higher than those in normal group(15.1±4.5)pg/ml vs.(12.8±3.7)pg/ml,5.2±1.8vs.2.6±0.4,P<0.05). Conclusion Moderate intensity exercise training can improve learning and memory ability of adolescent rats,which may be related to the increase of serum leptin content.