益气活血祛痰方对非酒精性脂肪肝模型小鼠血脂、动脉粥样硬化指数和冠心指数的影响

Effects of Yiqi Huoxue Qutan Compound on Blood Lipid, Atherosclerosis Index and Coronary Index of Model Mice with Nonalcoholic Fatty Liver Disease

目的观察益气活血祛痰方对非酒精性脂肪肝模型小鼠血脂、动脉粥样硬化指数(AI1、AI2)和冠心指数(R-CHR)的影响,探讨其作用机制。方法 15只雄性LDLR-/-小鼠给予高脂饮食饲养12周后,随机分为模型组、中药组、西药组,每组5只,另取5只雄性C57BL/6J小鼠作为正常组。正常组和模型组给予生理盐水灌胃,中药组给予益气活血祛痰方灌胃,西药组给予辛伐他汀灌胃,每日1次,连续12周。称取各组小鼠体质量和肝脏质量并计算肝脏指数;全自动生化分析仪检测总胆固醇(TG)、三酰甘油(TC)、低密度脂蛋白胆固醇(LDL-C)和高密度脂蛋白胆固醇(HDL-C)含量,计算AI1、AI2和R-CHR;HE染色观察肝组织病理形态。结果与正常组比较,模型组小鼠体质量、肝脏质量和肝脏指数明显升高(P<0.01),TG、TC和LDL-C含量明显增加(P<0.05),HDL-C含量明显减少(P<0.05),AI1、AI2和R-CHR均明显升高(P<0.01);与模型组比较,中药组和西药组小鼠体质量、肝脏质量和肝脏指数均明显降低(P<0.05),TG、TC和LDL-C含量明显减少(P<0.05),HDL-C含量明显增加(P<0.05),AI1、AI2和R-CHR均明显降低(P<0.01);HE染色结果显示,正常组小鼠肝脏组织染色正常,模型组出现脂肪变性,形成不同程度的脂肪滴;与模型组比较,中药组与西药组小鼠肝脏组织病变程度明显减轻。结论益气活血祛痰方具有调节脂质代谢紊乱和改善非酒精性脂肪肝的作用。

Objective To observe the effects of Yiqi Huoxue Qutan Compound on blood lipid,atherosclerosis index (AI1,AI2)and coronary index (R-CHR)of model mice with nonalcoholic fatty liver disease;To discuss its mechanism of action.Methods Fifteen male LDLR^-/-mice were given a high-fat diet for 12 weeks,and then were randomly divided into model group,TCM group and Western medicine group,with 5 mice in each group. Meanwhile,5male C57BL/6J mice were set as normal group.Normal group and model group were given saline for gavage;TCM group was given Yiqi Huoxue Qutan Compound for gavage;Western medicine group was given Simvastatin,once a day for 12 weeks.The body mass and liver mass of each group were measured and the liver index was calculated.The content of total cholesterol (TC),triglyceride (TG),high-density lipoprotein cholesterol (HDL-C)and low-density lipoprotein cholesterol (LDL-C)were detected by automatic biochemical analyzer,and AI1,AI2and R-CHR were calculated.HE staining was used to observe the pathological changes of liver tissue. Results Compared with normal group,the body mass,liver mass and liver index of the mice significantly increased (P<0.01);the contents of TG,TC and LDL-C increased (P<0.05);the content of HDL-C decreased (P<0.05);AI1,AI2 and R-CHR significantly increased (P<0.01)in the model group.Compared with model group,the body mass, liver mass and liver index of the mice significantly decreased (P<0.05);the contents of TG,TC and LDL-C decreased (P<0.05);the content of HDL-C content increased (P<0.05);AI1,AI2 and R-CHR significantly decreased (P<0.01)in TCM group and Western medicine group.The results of HE staining showed that the liver tissues of mice in the normal group were normal;fatty degeneration and fat droplets in different degrees were formed in the model group;the degree of disease was significantly reduced in the TCM group and Western medicine group. Conclusion Yiqi Huoxue Qutan Compound has the effect of regulating lipid metabolism disorder and improving nonalcoholic fatty liver disease.