摘要
The catalytic properties and thermodynamic kinetics of the endoglucanase from a marine Bacillus subtilis were analyzed. Optimum pH and temperature of the endoglucanase activity were 5.0 and 35 °C. The endoglucanase activity, melt point temperature was 1.13 folds(247.02 U·ml^(-1)), 2.1 °C higher(39.2 °C) in 6% ethanol solution than that(218.60 U·ml^(-1)),(37.1 °C) in free ethanol. At 40 °C–55 °C, Gibbs free energy, ΔG, and the content ofα-helix was higher in 6% ethanol solution than that in ethanol free solution. The increasing of α-helix content led to higher activity and better thermostability in ethanol solution. The cold adapt ethanol tolerant endoglucanase was valuable for bioethanol product by simultaneous saccharification and fermentation process.
The catalytic properties and thermodynamic kinetics of the endoglucanase from a marine Bacillus subtilis were analyzed. Optimum pH and temperature of the endoglucanase activity were 5.0 and 35 °C. The endoglucanase activity, melt point temperature was 1.13 folds(247.02 U·ml^(-1)), 2.1 °C higher(39.2 °C) in 6% ethanol solution than that(218.60 U·ml^(-1)),(37.1 °C) in free ethanol. At 40 °C–55 °C, Gibbs free energy, ΔG, and the content ofα-helix was higher in 6% ethanol solution than that in ethanol free solution. The increasing of α-helix content led to higher activity and better thermostability in ethanol solution. The cold adapt ethanol tolerant endoglucanase was valuable for bioethanol product by simultaneous saccharification and fermentation process.
基金
Supported by the Science and Technology Research Project of Hubei Provicial Department of Education(B2017041)
