纳米金磁免疫层析在瘦肉精残留检测中的应用研究

APPLICATION STUDY OF THE RESIDUE DETECTION OF CLENBUTEROL BY IMMUNOCHROMATOGRAPHY METHOD WITH GOLD MAGNETIC NANOPARTICLES

为优化动物源性食品中低浓度瘦肉精定量检测,建立了以金磁微粒为反应载体的免疫层析检测系统。应用结果表明,目标物盐酸克伦特罗(CLB)、莱克多巴胺(RAC)试纸条标准曲线范围分别为0.5～50 ng/mL、1.0～100 ng/mL;CLB和RAC试纸条检测下限分别可达0.25 ng/mL和0.5 ng/mL,CLB和RAC与其他同类药物反应均呈阴性,特异性良好;各批添加回收率在82.71～104.12%之间,准确度达到要求;不同CLB、 RAC标准添加水平下变异系数(CV)均小于15%,符合精密度标准。样品中假阳性率和假阴性率均为0。该方法将试纸条应用于猪肉制品中CLB和RAC残留的快速检测,具有灵敏度高、稳定性好和特异性强的特点。

In order to optimize the quantitative determination of low concentration clenbuterol from the food of animal origin, a immunochromatography detection system was set up using gold magnetic nanoparticles as supports. The results showed that the standard curves of clenbuterol(CLB) and rctopamine(RAC) test trips were between 0.5~50 ng/mL and 1.0~100 ng/mL, respectively. The lower limits of detection(LLOD) were 0.25 ng/mL and 0.5 ng/mL.CLB and RAC react negatively with drugs of same kind. The recovery rate is between 82.71 and 104.12 %, which means the accuracy meets the requirements. Coefficient of variation(CV) is lower than 15 % with different CLB and RAC adding level, which means the the precision meets the requirements. The false positive rate and false negative rate were both 0.This method shows high sensitivity, stability and specificity for CLB and RAC detection.