JAK1/JAK2抑制剂外用对特应性皮炎小鼠模型的影响

Effects of Topical JAK1/JAK2 Inhibitor on Atopic Dermatitis Mice Model

目的观察JAK1/JAK2抑制剂外用对特应性皮炎(atopic dermatitis,AD)小鼠模型的影响并探讨其作用机制。方法将18只BALB/C小鼠随机分为正常组、模型组、治疗组。模型组及治疗组应用2,4-二硝基氯苯诱导AD模型后,皮损处分别涂抹不含JAK1/JAK2抑制剂的空白软膏、JAK1/JAK2抑制剂软膏,连续涂抹14天。正常组仅脱毛,不给予任何治疗。实验第28天肉眼观察各组小鼠背部皮损处表现;HE染色观察皮损组织病理形态;ELISA法检测血清IgE水平;RT-qPCR法检测皮损组织IL-4、IL-5、IFN-γ mRNA表达。结果治疗组背部皮损处表现及组织病理形态较模型组明显改善,血清IgE含量明显减低,皮损组织IL-4、IL-5、IFN-γ mRNA相对表达量明显减少。结论 JAK1/JAK2抑制剂外用对AD小鼠模型具有较好的治疗作用,其机制之一可能与抑制皮损组织IL-4、IL-5、IFN-γ mRNA表达相关。

Objective To observe the effect of topical Jak1/Jak2 inhibitor on atopic dermatitis (AD) mice model and to explore its mechanism. MethodsEighteen BALB/C mice were randomly divided into normal group,model group,and treatment group. After the model group and the treatment group were treated with 2,4-dinitrochlorobenzene to induce the AD model,the skin lesions were coated with a blank ointment containing no JAK1/JAK2 inhibitor and a JAK1/JAK2 inhibitor ointment for 14 days. The normal group only had hair removed and no treatment was given. On the 28th day of the experiment,the appearance of the skin lesions on the back of each group was observed;the histopathological changes of lesions was observed by HE staining;the serum IgE level was detected by ELISA,and the expressions of IL-4,IL-5 and IFN-γ mRNA in the lesions were detected by RT-qPCR. ResultsIn the treatment group,the appearance and histopathological changes of the lesions improved significantly,the serum IgE content decreased significantly,and the relative expression of IL-4,IL-5,IFN- γ mRNA in the lesions decreased significantly. ConclusionThe topical use of JAK1/JAK2 inhibitor has a good therapeutic effect on the AD mice model,and one of the mechanisms may be related to the inhibition of IL-4,IL-5 and IFN-γ mRNA expression in lesional tissues.