慢性自发性荨麻疹血清YKL-40的变化及其与病情的相关性

Changes of Serum YKL-40 in Patients with Chronic Spontaneous Urticaria and the Correlation with Disease Activity

目的探讨慢性自发性荨麻疹患者血清YKL-40(人软骨糖蛋白39)的变化及其与病情的相关性。方法采用酶联免疫法检测50例慢性自发性荨麻疹患者及30名健康志愿者血清YKL-40、IL-4、IL-10、IFN-γ水平。结果慢性自发性荨麻疹患者组血清YKL-40及TH2型细胞因子IL-4、IL-10水平明显高于对照组(P<0.05),TH1型细胞因子IFN-γ水平明显低于对照组(P<0.05);YKL-40水平与荨麻疹症状评分正相关(r=0.43,P<0.05),与病程不存在相关性(r=0.05,P>0.05);自体血清皮肤试验阳性患者血清YKL-40水平明显高于阴性者(P<0.05);慢性自发性荨麻疹患者组血清YKL-40的变化与IL-4变化正相关(r=0.43,P<0.05),与IL-10、INF-γ不相关(r=0.16,0.10,P>0.05)。结论慢性自发性荨麻疹患者外周血血清YKL-40高表达,且与病情正相关,可能参与慢性荨麻疹的发生发展。

Objective To explore serum YKL-40 level in patients with chronic spontaneous urticaria (CSU) and the correlation between serum YKL-40 and the disease activity. Methods Fifty patients with CSU and 30 healthy volunteers were grouped for the study. Serum YKL-40,IL-4,IL-10 and IFN-γ levels were measured by enzyme-linked immunosorbent assay (ELISA). ResultsThe levels of serum YKL-40 and TH2 cytokines such as IL-4 and IL-10 were significantly higher in the CSU group than in the control group (P<0.05),and the level of TH1 cytokine such as IFN-γ was remarkably lower in the CSU group than in the control group (P<0.05). The YKL-40 level showed positive correlation with urticaria activity score (UAS) (r=0.43,P<0.05),while there was no correlation between YKL-40 level and course of the disease (r= 0.05,P>0.05). The YKL-40 level was significantly higher in autoserum skin test (ASST) positive patients than in ASST negative ones (P<0.05). For the CSU group,the serum YKL-40 changes positively correlated with IL-4 changes (r=0.43,P< 0.05),yet unrelated to IL-10 or INF-γ (r=0.16 ,0.10;P>0.05). ConclusionHigh expression of YKL-40 in peripheral blood serum in patients with CSU positively correlates with urticaria activity score,and YKL-40 may participate in the occurrence and development of chronic urticaria.