多倍体鲫鲤原始生殖细胞的标记及其迁移研究

Visualization and Migration of Primordial Germ Cells in Polyploid Crucian Carp

原始生殖细胞(primordial germ cells, PGCs)的起源和迁移已在多种鱼类进行了研究,但多倍体鲫鲤原始生殖细胞的标记和迁移尚未见报道。本实验室前期通过远缘杂交方法成功获得了一个倍性明确、亲缘关系清晰的多倍体杂交鱼研究体系,该体系由异源四倍体鲫鲤及其二倍体父母本和三倍体杂交后代组成。本文利用RNA定点表达(localized RNA expression, LRE)技术,将来自于斑马鱼的nanos1-3'-UTR与GFP融合后生成的m RNA注射入多倍体鱼受精卵中,首次对多倍体鲫鲤原始生殖细胞进行标记,并观察了其迁移途径。结果显示,多倍体鲫鲤PGCs能被斑马鱼GFP-nanos1-3'-UTR标记,并且多倍体鲫鲤PGCs的迁移与斑马鱼类似。本实验结果为多倍体鲫鲤原始生殖细胞的产生和迁移的研究提供了一些基础数据。

The origin and migration of primordial germ cells (PGCs) have been studied in a variety of fish species, but the visualization and migration of PGCs in polyploid fish have not been reported. The unique fish lineages in our laboratory (including allotetraploid carp, red crucian carp and triploid crucian carp), which have clear genetic backgrounds, were used to label PGCs. In this paper, the mRNA generated by fusion of nanos1-3′-UTR and GFP from zebrafish was injected into the fertilized eggs of polyploid fish, and the PGCs of polyploid fish were labeled for the first time to observe their migration route to the genital ridge. The results showed that the zebrafish nanos1-3′-UTR could label the PGCs of polyploid fish and their migration was similar to those of zebrafish. These results provide some basic data for the study of the origin and migration of PGCs of polyploid fish.