豚鼠骨导前庭诱发肌源性电位实验方法的建立及正常值测试

Establishment of experimental method for bone-conducted vibration elicited vestibular-evoked myogenic potentials in healthy guinea pigs

目的通过骨导振动刺激(bone-conducted vibration,BCV)诱发健康豚鼠颈肌前庭诱发肌源性电位(cervical vestibular-evoked myogenic potentials,cVEMP)和眼肌前庭诱发肌源性电位(ocular vestibular-evoked myogenic potentials,oVEMP),从而建立稳定的豚鼠BCV-cVEMP/BCV-oVEMP实验方法并获取正常值参数。方法选取11只(22耳)健康豚鼠,在动物清醒状态下通过特制的固定架,保持其俯卧抬头位后行BCV-cVEMP、BCV-oVEMP测试,分析记录BCV-cVEMP和BCV-oVEMP的波形特点和参数。结果11只健康豚鼠中BCV-cVEMP和BCV-oVEMP的引出率均为100%(22/22)。BCV-cVEMP的阈值为(85.5±10.8)dBSPL,n1潜伏期为(4.5±1.3)ms,p1潜伏期为(5.8±1.4)ms,n1-p1波间期为(1.2±0.4)ms,振幅为(21.5±17.3)μV。BCV-oVEMP的阈值为(90.7±10.6)dBSPL,n1潜伏期为(4.3±1.5)ms,p1潜伏期为(5.6±1.7)ms,n1-p1波间期为(1.4±0.6)ms,振幅为(24.0±16.3)μV。结论本实验在健康豚鼠身上成功诱发出稳定的BCV-cVEMP和BCV-oVEMP波形,并获取阈值、潜伏期和振幅等参数。

Objective To estabilsh animal methods of bone-conducted vibration elicited cervical vestibular-evoked myogenic potentials (BCV-cVEMP) and ocular vestibular-evoked myogenic potentials (BCV-oVEMP) in healthy guinea pigs. Methods Eleven healthy (250-350 g) and awake guinea pigs were selected and undertake conventional BCV-cVEMP and BCV-oVEMP examination in prone position. Parameters of waveforms were cauculated. Results The BCV-cVEMP and BCV-oVEMP both could be elicited in 100% (22/22) in guinea pigs respectively, threshold was (85.5±10.8)dB SPL and (90.7±10.6)dB SPL for cVEMP and oVEMP;n1 latency was (4.5±1.3)ms and (4.3±1.5)ms for cVEMP and oVEMP;p1 latency was (5.8±1.4)ms and (5.6±1.7)ms respectively;n1-p1 interwave latency was (1.2±0.4)ms for cVEMP and (1.4±0.6)ms for oVEMP, amplitude was (21.5±17.3)μV and (24.0±16.3)μV respectively. Conclusion Both BCV-cVEMP and BCV-oVEMP can be successfully elicited in healthy guinea pigs.