钩藤人参合用对高血压合并心衰大鼠模型的影响

Effects of combination of Uncaria rhynchophylla and Panax ginseng on hypertension complicated with heart failure in rats

目的研究钩藤人参合用对高血压合并心衰(HCHF)模型大鼠的影响。方法将大鼠随机分为假手术组、模型组、卡托普利组(0.014 3 g/kg)和钩藤人参合用高(钩藤0.9 g/kg+人参1.8 g/kg)、中(钩藤0.45 g/kg+人参0.9 g/kg),低(钩藤0.225 g/kg+人参0.45 g/kg)剂量组。采用腹主动脉缩窄术制作大鼠HCHF模型,5周后通过大鼠尾动脉压监测仪评估造模是否成功,其中假手术组只分离腹主动脉,不结扎。酶联免疫吸附法(ELISA)测定大鼠血清B型脑钠肽(BNP)、血管紧张素II (Ang II)、血管紧张素1型受体(AT1)、β1肾上腺素受体(β1-AR)水平。取心脏测定心脏质量指数(HWI),HE染色法观察大鼠心肌纤维排列状况。结果造模第5周后手术各组收缩压较对照组显著升高(P<0.01),验证模型成功;给药第2、4周,钩藤人参合用中、高剂量组收缩压较模型组显著下降(P<0.05、0.01)。与模型组比较,钩藤人参合用中、高剂量组能显著降低心衰大鼠的HWI以及AT1、Ang II、BNP的浓度,显著升高β1-AR浓度(P<0.05、0.01),且呈剂量相关性。光镜下观察模型组心肌结构紊乱,心肌细胞排列呈波浪状,心肌纤维断裂严重;卡托普利组和人参钩藤高剂量组可见心肌结构排列较均匀,心肌细胞形态正常,心肌纤维略有断裂。结论钩藤人参合用对于降低大鼠血压,改善大鼠心衰症状效果显著,且呈剂量相关性,其中高剂量与卡托普利组效果相当。

Objective To study the effects of combination of Uncaria rhynchophylla and Panax ginseng on hypertension complicated with heart failure(HCHF)in rats.Method Rats were randomly divided into sham-operated group,model group,captopril group(0.0143 g/kg),high(Uncaria 0.9 g/kg+ginseng 1.8 g/kg),middle(Uncaria 0.45 g/kg+ginseng 0.9 g/kg),and low(Uncaria 0.225 g/kg+ginseng 0.45 g/kg)dose combination of Uncaria and ginseng groups.Rats with HCHF model was produced by abdominal aortic coarctation.After 5 weeks,assessing the success of the model through rat tail artery pressure monitoring.In the sham operation group,only abdominal aortas of rats were isolated without ligation.Enzyme-linked immunosorbent assay(ELISA)was used for determination of rat serum BNP,AngⅡ,AT1,and β1-AR.Cardiac quality index(HWI)was measured by heart test,and myocardial fiber arrangement was observed by HE staining.Results Systolic blood pressure(SBP)was significantly higher in each group than that in the control group(P<0.01)after the 5th week of model establishment,and the model was verified to be successful.At the 2nd and 4th week of administration,the SBP of the medium and high dose groups of combination of Uncaria and ginseng was significantly lower than that of the model group(P<0.05,0.01).Compared with the model group,each dose group of combination can significantly reduce the HWI and the concentration of AT1,AngⅡ,and BNP in rats with heart failure(P<0.05),and significantly increaseβ1-AR concentration(P<0.05)in a dose-dependent manner.Under light microscope,we found that the myocardial structure of the model group was disordered,the myocardial cells arranged in a wavy manner,and the myocardial fiber was severely broken.In the captopril group and the high-dosecombination group,the myocardial structure was arranged more evenly,the myocardial cell morphology was normal,and the myocardial fiber was slightly broken.Conclusion Combination of Uncaria and ginseng has a significant effect on lowering blood pressure and improving symptoms of heart failure in rats.Among them,the high-dose combination group has the best effect,and has the same effect as the captopril group.