金钗石斛破壁粉对环磷酰胺致免疫低下小鼠免疫功能的调节作用

Effects of Dendrobium nobile wall-broken powder on immune function in immunosuppression mice induced by cyclophosphamide

目的研究金钗石斛破壁粉对免疫低下小鼠免疫功能的影响。方法将180只小鼠随机分为6组,每组30只,分别为对照组、模型组、左旋咪唑(阳性对照,ig给予盐酸左旋咪唑片0.025 g/kg)组及金钗石斛破壁粉低、中、高剂量(0.5、1.0、2.0 g/kg)组。除对照组外,其余5组于给药前3 d ip 75 mg/kg新配制的环磷酰胺,每天1次,连续3 d,制备免疫功能低下小鼠模型。造模完成后每天ig给药1次,对照组和模型组给予等体积的生理盐水,连续14 d。通过计算脾指数与胸腺指数、腹腔巨噬细胞吞噬鸡红细胞实验、血清溶血素测定、淋巴细胞转化试验及western blotting检测脾脏中细胞因子IL-2、TNF-α的蛋白表达,观察金钗石斛破壁粉对免疫低下小鼠免疫功能的调节作用。结果与模型组比较,金钗石斛破壁粉1.0、2.0 g/kg显著升高免疫低下小鼠的脾指数与胸腺指数,提高巨噬细胞吞噬功能,升高血清溶血素含量,促进淋巴细胞转化反应,上调小鼠脾脏中细胞因子白细胞介素-2(IL-2)、肿瘤坏死因子-α(TNF-α)的蛋白表达(P<0.05、0.01)。结论金钗石斛破壁粉显著增强免疫低下小鼠的免疫功能。

Objective To observe the effects of Dendrobium nobile Lindl.wall-broken powder(DNLP)on immune function in immunosuppression mice induced by cyclophosphamide.Methods A total 180 mice were randomly divided into six groups,30 mice in each group,namely control group,model group,levamisole(positive control,ig 0.025 g/kg levamisole hydrochloride tablets)group and low,medium and high dose(0.5,1.0,2.0 g/kg)group of DNLP.Except for the control group,the other five groups were ip given 75 mg/kg of cyclophosphamide for three days before administration,once a day for three days to prepare immunodeficient mice model.Mice was ig administered once a day after the completion of the model for 14 consecutive days.The control group and the model group were given saline of the same volume.The effect of DNLP was investigated by spleen index and thymus index,experiment of peritoneal macrophages phagocytizing chicken red blood cells,serum hemolysin test,T lymphocyte proliferation test,and Western blotting to detect IL-2 and TNF-α protein expression of spleen in mice.Results Compared with the model group,1.0g/kg and 2.0g/kg DNLP significantly increased the spleen index,thymus index,and the content of serum hemolysin,improved the phagocytosis of macrophage,and enhanced the T lymphocytes proliferation,and IL-2 and TNF-α protein expression of spleen in immunosuppression mice(P<0.05 and 0.01).Conclusion DNLP can significantly improve the immune function in immunosuppression mice.