扶正解毒化瘀颗粒对多器官损伤小鼠血清细胞因子的影响

Effect of Fuzheng Jiedu Huayu Granule on serum cytokines of multi-organ injury in mouse

目的观察扶正解毒化瘀颗粒对克雷伯杆菌肺炎所致多器官损伤小鼠不同感染时相血清TNF-α、IL-1β、IFN-γ、IL-6及IL-10水平的动态变化,探讨其对促炎/抗炎细胞因子表达的影响。方法将128只小鼠随机分为对照组、模型组、西药组和中药组,每组32只。采用气管插管法直接注入肺炎克雷伯杆菌制备多器官损伤模型,分别于造模后第1、3、5、7天每组各取8只小鼠,摘眼球采集血液,分离血清。采用ELISA法,检测不同感染时相小鼠血清中细胞因子TNF-α、IL-1β、IFN-γ、IL-6及IL-10水平。结果与对照组比较,模型组小鼠在感染后第1天血清TNF-α、IL-1β、IFN-γ和IL-6含量分别为(202.07±22.82)、(502.11±29.44)、(186.50±10.32)、(204.98±8.15)pg/mL,其含量水平显著增高,差异有统计学意义(P<0.05)。IL-10在感染后第1、3、5天含量有所增高,但差异无统计学意义(P>0.05),在感染后第7天含量为(134.34±0.46)pg/mL,差异有统计学意义(P<0.05);中药组小鼠在感染后第1天血清TNF-α、IL-1β、IFN-γ和IL-6含量分别为(147.18±22.67)、(262.09±43.44)、(142.00±0.84)、(177.29±8.03)pg/mL,均显著低于模型组,差异有统计学意义(P<0.05),在感染第3天后有所下降,但差异无统计学意义(P>0.05)。感染后第1、7天血清IL-10含量为(140.46±4.07)pg/mL和(136.48±0.10)pg/mL均显著增高,有统计学意义(P<0.05);西药组小鼠在感染后第1天血清IL-6的含量(180.97±8.86)pg/mL、IFN-γ含量(144.39±14.67)pg/mL较模型组均显著降低,差异有统计学意义(P<0.05)。结论扶正解毒化瘀颗粒能够降低促炎细胞因子的表达,提高抗炎细胞因子的表达,通过调节促炎/抗炎细胞因子的表达,抑制机体炎症反应可能是其治疗多器官损伤的主要机制之一。

Objective To explore the effect of Fuzheng Jiedu Huayu Granule on pro-inflammatory/anti-inflammatorycytokine of multiple organ injury induced by Klebsiella pneumoniae, through the dynamic changes of serum TNF-α, IL-1β,IFN-γ, IL-6, and IL-10 levels in different infection phases of mice. Methods A total of 128 KM mice were randomlydivided into control group, model group, western medicine group and traditional Chinese medicine group with 32 animals ineach group. The multi-organ injury model was prepared by direct injection of Klebsiella pneumoniae by tracheal intubationmethod. Eight mice were sacrificed on the 1st, 3rd, 5th, and 7th days after modeling respectively;the eyeball were taken outand collecting blood and separating serum. The levels of TNF-α, IL-1β, IFN-γ, IL-6, IL-10 in serum of mice with differentinfection phases were observed by ELISA. Results Compared with control group, serum levels of TNF-α, IL-1β, IFN-γ andIL-6 in model group on the 1st day after infection were(202.07±22.82),(502.11±29.44),(186.50±10.32),(204.98±8.15) pg/mL, significantly increased(P<0.05), after the 3th day of infection, there was a gradual downward trend, and the difference wasnot statistically significant on the 7th day(P >0.05), the content of IL-10 on the 7th day after infection was(134.34±0.46) pg/mL(P<0.05), and the content increased on the 1st, 3th and 5th days after infection(P >0.05). The contents of serum TNF-α, IL-1β, IFN-γ and IL-6 in the Chinese medicine group on the 1st day after infection were(147.18±22.67),(262.09±43.44),(142.00±0.84),(177.29±8.03) pg/mL, both were significantly lower than the model group(P<0.05), and decreased after the 3thday of infection(P >0.05), the serum IL-10 content on the 1st day(140.46±4.07) pg/mL and on the 7th day(136.48±0.10) pg/mL after infection were increased significantly(P<0.05), the serum IL-6 content(180.97±8.86) pg/mL and IFN-γ content(144.39±14.67) pg/mL in the western medicine group were significantly lower than those in the model group on the 1st day afterinfection(P<0.05). Conclusion Fuzheng Jiedu Huayu Granule can reduce the expression levels of pro-inflammatorycytokines and improve the expression level of anti-inflammatory cytokines in serum, by inhibiting the inflammatory response ofbody through regulating expression of pro-inflammatory/anti-inflammatory cytokines, it may be one of the main mechanisms forthe treatment of multi-organ injury.