miR-346在结肠癌细胞中的表达及功能研究

Expression and function of miR-346 in colon cancer cells

目的检测结肠癌细胞中miR-346表达及侵袭转移的关系,为结肠癌预测及治疗靶点提供试验依据。方法采用qRT-PCR法(quantificational real-time polymerase chain reaction)检测结肠癌细胞株(HCT116、COLO205、SW620)及正常结肠上皮细胞(NCM460)中miR-346的表达。采用miR-346拟似剂(mimic)或抑制剂(inhibitor)对miR-346表达进行上调或下调并转染HCT116细胞,qRT-PCR检测转染效率,Transwell观察转染后HCT116细胞转移及浸润行为变化。结果miR-346在结肠癌细胞株HCT116、COLO205、SW620中相对表达量分别为0.372±0.068、1.284±0.253、1.105±0.203,在正常结肠上皮细胞(NCM460)中相对表达量为0.126±0.004,差异有统计学意义(F值=4.563,P<0.05),与HCT116组比较,COLO205组及SW620组差异有统计学意义(t值分别为7.256、5.419,P均<0.01)。与NCM460组比较,COLO205组及SW620组差异有统计学意义(t值分别为9.652、7.753,P均<0.01)。miR-346表达上调后HCT116细胞侵袭及转移能力增强(t值分别为2.458、2.194,P均<0.05),miR-346表达下调后HCT116细胞侵袭及转移能力减弱(t值分别为2.247、2.065,P均<0.05)。结论miR-346在结肠癌细胞中表达高,调控结肠癌细胞侵袭及转移。

Objective To detect the expression of miR-346 in colon cancer cells and the relationship with invasion and metastasis, so as to provide experimental basis for predicting and treating targets of colon cancer.Methods Quantitative real-time polymerase chain reaction (qRT-PCR) was used to detect the expression of microRNA-346 in colon cancer cell lines (HCT116, COLO205, SW620) and normal colon epithelial cells (NCM460). The expression of microRNA-346 was up-regulated or down-regulated by mimic or inhibitor and transfected into HCT116 cells.The transfection efficiency was detected by qRT-PCR.The metastasis and infiltration behavior of HCT116 cells were observed by Transwell.Results The relative expression of microRNA-346 in colon cancer cell lines HCT116, COLO205 and SW620 were (0.372±0.068), (1.284±0.253), (1.105±0.203), respectively.The relative expression of COLO-205 and SW620 in normal colon epithelial cells (NCM460) was (0.126±0.004). Compared with NCM460 group, COLO-205 group and SW620 group had significant difference (t=9.652, P<0.01;t=7.753, P<0.01). The invasion and metastasis ability of HCT116 cells increased after the up-regulation of the expression of microRNA-346 (t=2.458, P<0.05;t=2.194, P<0.05), and decreased after the down-regulation of the expression of microRNA-346 (t=2.247, P<0.05;t=2.065, P<0.05).Conclusion Mir-346 is highly expressed in colon cancer cells and regulates invasion and metastasis of colon cancer cells.