摘要
目的探讨纳米珍珠粉(nano-pearl powder,NPP)对小鼠骨髓间充质干细胞活性的影响。方法研究于2016年8月至2017年3月在中南大学湘雅医学院附属海口医院实验中心进行。将NPP以100、300、500μg/mL的质量浓度与小鼠骨髓间充质干细胞共培养24 h(NPP组),用CCK8法测定吸光度值,计算细胞相对增殖率,评定其细胞毒性级别;用Annexin-FITC/PI法检测细胞凋亡率。两者综合评定NPP对小鼠骨髓间充质干细胞活性的影响,并与相同质量浓度纳米羟基磷灰石(nano-hydroxyapatite,NHA)(NHA组)进行对比。结果 NPP与NHA细胞毒性检测均合格,NPP在100μg/mL时细胞相对增殖率最高;NPP组细胞凋亡率均低于NHA组,在质量浓度为100和500μg/mL时的差异有统计学意义(P <0.01)。结论 NPP细胞毒性检测合格,其对小鼠骨髓间充质干细胞活性的影响优于NHA。
Objective To investigate the effect of nano-pearl powder(NPP)on cell viability of mouse bone marrow mesenchymal stem cells. Methods Mouse bone marrow mesenchymal stem cells(m BMMSCs)were co-cultured with NPP at concentration of 100,300,500 μg/mL for 24 h(NPP group). CCK8 test was carried out to evaluate the relative growth rate and cytotoxicity. The apoptosis rate of cells was measured by Annexin-FITC/PI method. The effect of NPP on mBMMSCs activity was analyzed by the above two approaches,and the results were compared with nano-hydroxyapatite(NHA group)at the same concentration. Results The cytotoxicity tests of NPP and NHA were qualified,and the relative proliferation rates of mBMMSCs in NPP groups were highest at 100 μg/mL.The apoptotic rates of mBMMSCs in NPP groups were all lower than those in NHA groups. The difference was statistically significant at 100 and 500 μg/mL(P<0.01). Conclusion The cytotoxicity tests of NPP are qualified,and its effect on the cell viability of mBMMSCs is better than that of NHA.
作者
郑斌
徐普
李晓妮
陈灵
程亚楠
张文柏
王秋子
ZHENG Bin;XU Pu;LI Xiao-ni;CHEN Ling;CHENG Ya-nan;ZHANG Wen-bo;WANG Qiu-zi(Department of Oral Implant,Affiliated Haikou Hospital,Xiangya Medical School,Central South University,Hainan Provincial Stomatology Center,Haikou 570208,China)
出处
《中国实用口腔科杂志》
CAS
2019年第1期26-30,共5页
Chinese Journal of Practical Stomatology
基金
海南省重点研发项目(ZDYF2016018)
海口市重点科技计划项目(2013-58)
