火焰南天竹组织培养体系建立与优化

Establishment and Optimization of Tissue Culture System for Nandina domestica 'Fire power'

火焰南天竹(Nandina domestica‘Fire power’.)属于小檗科(Berberidaceae)南天竹属(Nandina)的常绿小灌木,在园林应用中具有十分重要的观赏价值。常规育苗方法扦插繁殖,繁殖率低,火焰南天竹组培中繁殖率及生根仍是一大难题。研究了不同激素对火焰南天竹不定芽诱导、增殖、生根的影响。结果表明:最佳诱导培养基为MS+0.3 mg/L 6-BA+30 g/L蔗糖,诱导率为93%;增殖培养基为MS+1.0 mg/L 6-BA+0.1 mg/L NAA+30 g/L蔗糖,增殖系数为3.5;生根培养基为1/2MS+0.3 mg/L IBA+20 g/L蔗糖,诱导率达97%。火焰南天竹种苗经有效成分含量为50%的多菌灵稀释1 000倍,浸泡1 h后移栽,移栽成活率高,长势良好。所建立的火焰南天竹快繁体系增殖速度快,短期内可获得大量植株,为工厂化生产提供技术支持,满足市场需求。

Common nandina(Nandina domestica'Fire power'.)belongs to Nandina genus,Berberidaceae,and it is the evergreen shrub with important ornamental value in landscape application.Traditional cultivation depends on cottage propagation,but reproduction rate is low,reproductive rate and rooting are still major problems in tissue culture of Nandina domestica 'Fire power'.In this research,effects of different hormones on adventitious buds induction,multiplication and rooting of Nandina domestica'Fire power'were studied.The results showed that MS+0.3 mg/L 6-BA+30 g/L sugar was the best induced medium,the induction rate was 93%;the subculture medium for differentiation was MS supplemented added with 1.0 mg/L 6-BA,0.1 mg/L NAA and 30 g/L sugar,the multiplication efficient was 3.5.The optimal rooting medium was 1/2 MS added with 0.3 mg/L IBA and 20 g/L sugar,the rooting rate of plantlets could reach 97%.The seedlings soaked in carbendazim with an effective component content of 50%diluted 1 000 times for 1 h showed high survival rate and good growth.The established propagation system of Nandina domestica'Fire power'had fast propagation rate,and a large number of plants could be obtained in the short term.This propagation technique provided technical support for factory production and meets the market demand.