裸仁南瓜籽油活性成分分析及抗氧化能力评价

Study on bioactive components and antioxidant activity of hull-less pumpkin seed oil

以超临界CO2萃取的裸仁南瓜籽油为研究对象,根据LS/T 3250—2017《南瓜籽油》标准检测其质量指标,运用气相色谱—质谱(GC-MS)联用仪分析脂肪酸和植物甾醇组分,高效液相色谱(HPLC)仪测定角鲨烯含量;以葡萄籽油和亚麻籽油作为对比,研究裸仁南瓜籽油对DPPH、ABTS自由基的清除能力,以评价其抗氧化活性。结果表明,超临界CO2萃取的裸仁南瓜籽油符合LS/T 3250—2017标准,裸仁南瓜籽油主要含有4种不饱和脂肪酸,总含量为74.73%,其中顺式单不饱和脂肪酸和多不饱和脂肪酸含量分别为29.14%,45.54%;富含植物甾醇,β-谷甾醇含量为30.75%,△-7植物甾醇含量达10.77%;角鲨烯含量为5 490μg/g,高达葡萄籽油的80倍,亚麻籽油的279倍;裸仁南瓜籽油具有良好的抗氧化活性,其抗氧化性与油浓度呈正相关,对DPPH自由基的清除能力(IC50为16.14 mg/mL)优于葡萄籽油、亚麻籽油,对ABTS自由基的清除能力(IC50为10.46mg/mL)优于亚麻籽油,裸仁南瓜籽油良好的抗氧化活性可能与其丰富的角鲨烯、植物甾醇和不饱和脂肪酸有关。

The quality indexes of hull-less pumpkin seed oil,which were extracted by supercritical CO2 fluid extraction,were detected according to the national standard.The fatty acid and phytosterol composition were analyzed by gas chromatography-mass spectrometry(GC-MS).Subsequently,the squalene content of hull-less pumpkin seed oil was measured by high performance liquid chromatography(HPLC).Compared with grape seed oil and flaxseed oil,the scavenging activities for DPPH· and ABTS free radical of hull-less pumpkin seed oil were estimated.The results indicated that the hullless pumpkin seed oil extracted by supercritical CO2 fluid extraction was according with the food industry standard LS/T 3250-2017《pumpkin seed oil》.The hull-less pumpkin seed oil mainly contained4 kinds of unsaturated fatty acid with a total content of 74.73%,including the cis-monounsaturated fatty acids and polyunsaturated fatty acids,with the content of 29.14%,45.54%,respectively.The contents ofβ-sitosterol and△-7 phytosterol were 30.75%,10.77%,respectively.Squalene in hull-less pumpkin seed oil was 5 490μg/g,up to 80 times of grape seed oil and 279 times of flaxseed oil.The antioxidant activities of hull-less pumpkin seed oil were positively correlated with its concentration.For the hull-less pumpkin seed oil,its ability to scavenge DPPH· free radical(IC5016.14 mg/mL)was better than that of grape seed oil and flaxseed oil,and the ability of scavenging ABTS free radical was equivalent to that of grape seed oil and flaxseed oil.Its good antioxidant activity was related to its rich squalene,phytosterol and unsaturated fatty acid.