摘要
以AB-8吸附树脂优化酸浆茎叶黄酮精制工艺,并评价其体外和体内抗氧化活性。试验结果表明,以70%的乙醇水溶液为洗脱剂,洗脱流速2mL/min,酸浆茎叶黄酮纯度可达39.16%。精制酸浆茎叶黄酮对NO-2·和ABTS+·的清除率分别为81.36%和91.49%,对亚油酸脂质过氧化抑制率为85.26%;精制酸浆茎叶黄酮不仅增加了小鼠的胸腺系数和脾脏系数(P<0.05),而且明显提高了血清T-AOC、GSH-Px和SOD活性(P<0.05),降低MDA含量(P<0.01),说明精制酸浆茎叶黄酮具有较强的体外和体内抗氧化活性。
The flavonoids from stems and leaves of Physalis alkekengi(PSF)were refined by macroporous adsorption resin,and their antioxidant activities were evaluated in vitro and in vivo.The PSF were obtained using with AB-8 macroporous adsorption resin washed by70% ethanol,and the purity was 39.16%.After refining,the scavenging activities of PSF on NO^-2·and ABTS+· were 81.36% and91.49%.The anti-oxidative capability in linoleic acid lipid overoxidation system was 85.26%.The refined PSF not only significantly increased thymus index and spleen index(P<0.05),but also increased the activity of T-AOC,GSH-Px and SOD in serum(P<0.05)and decreased the content of MDA in serum(P<0.01).The refined PSF showed strong antioxidant activity in vitro and in vivo.
作者
李群
孙明哲
吴威
姜秀娟
曹柏营
LI Qun;SUN Ming-zhe;WU Wei;JIANG Xiu-juan;CAO Bai-ying(Food and Biological Technology College,Changchun Vocational Institute of Technology,Changchun,Jilin 130033,China;Food Engineering College,J ilin Engineering Normal University,Changchun ,Jilin130052,China)
出处
《食品与机械》
CSCD
北大核心
2018年第10期158-162,共5页
Food and Machinery
基金
吉林省教育厅十二五科技规划项目(编号:吉教科合字2015第391号)
关键词
酸浆
黄酮
精制
大孔吸附树脂
抗氧化活性
Physalis alkekengi
Flavonoids
Refining
Macroporous adsorption resin
Antioxidant activity