基于~1H-NMR的干寒性气质失调证糖尿病大鼠肝组织代谢变化研究

Changes of Liver Metabolism In Diabetic Rats With Dry-Cold Temperament Disorder Based on ~1H-NMR

目的:观察干寒性气质失调证糖尿病模型大鼠肝脏组织的代谢变化。方法:对模型肝脏组织进行核磁共振检测,并对其代谢轮廓变化进行分析。结果:与健康组比较,干寒性气质失调证大鼠肝组织中β-羟基丁酸、肌酸、氧化三甲胺、苏氨酸和胆碱等代谢物含量明显升高,而乳酸、肌酸肝、葡萄糖和肌醇等含量明显降低;干寒性气质失调糖尿病大鼠肝组织中β-羟基丁酸、肌酸、肌醇和葡萄糖等含量明显升高,但异亮氨酸、亮氨酸、缬氨酸、乳酸、丙氨酸、鸟氨酸、瓜氨酸、蛋氨酸、谷氨酰胺、谷氨酸、α-酮戊二酸、乙酰天冬氨酸、甲胺、肌氨酸、肌酸肝、组氨酸、色氨酸、甘氨酸、苏氨酸、胆碱、酪氨酸、苯丙氨酸、烟酸和马尿酸等含量均明显降低。结论:干寒性气质失调证的发生伴随有多种能量代谢的变化。

Objective: To investigate the metabolic changes in liver tissue of Cold-Dry syndrome with type 2 diabetes(T2DM) rats. Methods: The liver tissues of Cold-Dry syndrome with T2 DM rats tested by ~1H-NMR spectroscopy. Results: Compared with healthy control group, Cold-Dry syndrome group has high concentration of β-hydroxy butyric acid, creatine, trimethylamine oxide, threonine and choline, but the content of lactic acid, glucose, creatinine and inositol concentration decreased significantly;the Cold-Dry syndrome with T2 DM has high concentration of β-hydroxy butyric acid, creatine, myo-inositol and glucose, but has lower concentration of isoleucine, leucine, valine, lactate, alanine, ornithine, citrulline, methionine, glutamine, glutamic acid, ketoglutaric acid, acetyl aspartic acid, dimethylamine, creatine, creatinine, histidine, tryptophan, glycine, threonine, tyrosine, phenylalanine, choline, nicotinic acid and hippuric acid. Conclusion: Various energy metabolism changes are related to Cold-Dry syndrome.