芒果苷对高尿酸血症小鼠血尿酸及嘌呤代谢相关酶表达的影响

Effect of Mangiferin on mRNA Expressions of Hepatic PRPS,PRPPAT and Cerebral HGPRT in Hyperuricemic Mice Induced by Potassium Oxonate

目的:研究芒果苷对氧嗪酸钾诱导的高尿酸血症小鼠血尿酸的影响以及对磷酸核糖焦磷酸合成酶(PRPS),磷酸核糖焦磷酸酰胺转移酶(PRPPAT)和次黄嘌呤-鸟嘌呤磷酸核糖转移酶(HGPRT) mRNA表达水平的影响。方法:雄性昆明种小鼠60只,随机分为正常组、高尿酸血症模型组、芒果苷1. 5,3. 0,6. 0 mg·kg^-1组、别嘌醇组(1. 0 mg·kg^-1),每组10只。按10 m L·kg-1每天灌胃给药2次,连续10次,给药后1 h再灌胃给予氧嗪酸钾(500. 0 mg·kg^-1)诱导形成高尿酸血症小鼠。磷钨酸法测血清尿酸水平,采用逆转录聚合酶链式反应(RT-PCR)检测小鼠肝脏PRPS,PRPPAT和脑组织中HGPRT mRNA的表达水平。结果:灌胃给予氧嗪酸钾后,与正常组比较,模型组小鼠血尿酸水平显著升高(P <0. 05);给予芒果苷和别嘌醇后,与模型组比较,芒果苷各剂量(1. 5,3. 0,6. 0 mg·kg^-1)和别嘌醇(1. 0 mg·kg^-1)均能显著降低高尿酸血症小鼠血清尿酸水平(P <0. 05,P <0. 01);与正常组和模型组比较,芒果苷各剂量组小鼠肝脏PRPS和PRPPAT mRNA的表达没有显著性差异,小鼠脑组织中HGPRT mRNA表达水平也没有显著性差异。结论:在本实验条件下,芒果苷的降尿酸作用与嘌呤代谢相关酶PRPS,PRPPAT和HGPRT mRNA的表达无关。

Objective: To investigate the effect of mangiferin on the mRNA expression of phosphoribosylpyrohoosphate synthetase( PRPS),phosphate ribose pyrophosphate amide transferase( PRPPAT) in liver and hypoxanthine-guanine phosphate transfer enzyme( HGPRT) in brain of hyperuricemic mice induced by potassium oxonate. Method: Hyperuricemic mice were induced through intraperitoneal injection with uricase inhibitor potassium oxonate. The serum uric acid level was determined by the phosphotungstic acid method. The mRNA expression levels of PRPS and PRPPAT in liver as well as HGPRT in brain of hyperuricemic mice were measured by reverse transcriptase polymerase chain reaction( RT-PCR). Result: An intraperitoneal injection with potassium oxonate caused a marked increase in serum uric acid level,compared with normal control group( P <0. 05). Intragastric administration with mangiferin at the doses of 1. 5,3. 0,6. 0 mg·kg^-1 was able to significantly reduce serum uric acid levels,compared with hyperuricemic control group( P < 0. 05,P < 0. 01);Moreover,the increase in the mRNA expression levels of PRPS and PRPPAT in liver,as well as the decrease in the mRNA expression of HGPRT in brain were observed in hyperuricemic control group,but with no statistically difference.Mangiferin has no impact on the mRNA expressions of PRPS,PRPPAT and HGPRT,compared with hyperuricemic control group. Conclusion: The hyporuricemic effect of mangiferin might not be related with PRPS,PRPPAT and HGPRT in therapeutic dose.