基于整合药理学平台探究半夏白术天麻汤治疗高血压的分子机制

Molecular Mechanism of Banxia Baizhu Tianma Tang in Treating Hypertension Based on Integrated Pharmacological Platform

目的:探讨半夏白术天麻汤治疗高血压的分子机制。方法:综合利用中药整合药理学平台(TCM-IP)的中药方剂数据库、中药材数据库、中药成分数据库和疾病/症状靶标数据库的信息和分析功能,检索方中中药所含的化学成分信息,构建半夏白术天麻汤作用的潜在靶标和疾病靶标之间的相互作用网络,然后富集计算核心靶标,进行基因功能分析和通路分析,再进一步构建"中药材-化学成分-核心靶标-关键通路"多维关系网络。结果:对半夏白术天麻汤预测共得到90个活性成分,包括生物碱、核苷、黄酮、皂苷、有机酸等;涉及287个核心靶标,其中包括13个直接靶标,如腺苷酸环化酶,G蛋白偶联β1受体,葡萄糖激酶等;涉及神经系统、内分泌系统、循环系统、雌激素信号通路、趋化因子信号通路、促性腺激素释放激素信号通路等相关生物过程和信号通路。结论:半夏白术天麻汤通过调节神经递质浓度和活性异常、改善胰岛素抵抗、调节炎症因子的产生、抑制炎症反应等过程来发挥保护血管内皮细胞的药理作用,进而达到治疗高血压的临床疗效,为全面阐释该方的作用机制提供了参考。

Objective: To explore the molecular mechanism of Banxia Baizhu Tianma Tang in treating hypertension. Method: Based on an internet-based computation platform for integrated pharmacology of traditional Chinese medicine( TCM),TCM prescription database,TCM database,TCM component database and disease/symptoms target database, information on the chemical compositions contained in TCM was retrieved, an interaction network between potential targets and disease targets of Banxia Baizhu Tianma Tang was built,then core target was enriched and calculated,gene function and pathway analysis was carried out,the multi-dimensional relationship network of "Chinese herbs-ingredients-critical targets-key pathways"of Banxia Baizhu Tianma Tang was further constructed. Result: A total of 90 active ingredients in Banxia Baizhu Tianma Tang were obtained,including alkaloids,nucleosides,flavonoids,saponins,organic acids and other ingredients;they involved 287 core targets,including 13 direct targets,such as adenylate cyclase,G protein coupled with β1 receptor,glucose kinase,etc;they also involved nervous system, endocrine system, circulatory system, estrogen signaling pathway,chemokine signaling pathway and other related biological processes and signaling pathways. Conclusion:Banxia Baizhu Tianma Tang can regulate neurotransmitter concentration and activity abnormalities,improve the protective effect of vascular endothelial cells by improving insulin resistance, regulating the production of inflammatory factors, and inhibiting inflammatory reactions, thereby exerting pharmacological effects on the treatment of hypertension. This study can provide a scientific basis for comprehensive interpretation of mechanism of Banxia Baizhu Tianma Tang.