十二烷基苯磺酸钠与盐酸肼屈嗪作用的荧光光谱分析研究及应用

Fluorescence Spectroscopic Analysis and Application of Sodium Dodecyl Benzene Sulfonate and Hydralazine Hydrochloride

基于盐酸肼屈嗪与十二烷基苯磺酸钠在Clark-Lubs缓冲介质中发生反应,建立了荧光光谱法测定盐酸肼屈嗪的新方法,对实验条件进行充分优化,结果发现,荧光光谱法的激发波长和发射波长分别为233 nm和290 nm。该实验方法测得盐酸肼屈嗪浓度在0. 5~4. 0 mg/L范围内呈现线性关系,其线性回归方程为ΔIF=765. 05c+161. 07,相关系数R为0. 999 6,检测限为0. 18 mg/L,回收率在99. 08%~101. 08%之间,该盐酸肼屈嗪含量测定方法与硼酸催化茚三酮分光光度法,所测结果相一致。

Based on the reaction between hydralazine hydrochloride and sodium dodecyl benzene sulfonate in Clark-Lubs buffer medium,a new fluorescence spectrometry method for the determination of hydralazine hydrochloride was established. The experimental conditions were fully optimized.The excitation and emission wavelengths of the fluorescence spectrum were 233 nm and 290 nm,respectively.The hydralazine hydrochloride concentration showed a linear relationship in the range of 0. 5 ~ 4. 0 mg/L,and the linear regression equation was ΔIF= 765. 05 c+161. 07 with the correlation coefficient 0. 999 6,the detection limit 0. 18 mg/L,and the recovery rate of between 99. 08% ~ 101. 08%.The hydralazine hydrochloride content determination of this method is consistent with the results measured by boric acid catalyzed ninhydrin spectrophotometry.