丁香蘑菇野生菌株的生物学特性及驯化栽培

Biological Characteristics and Domestication Cultivation of Wild Edible Fungus Agaricus padanus

以采自内蒙古鄂尔多斯市乌审旗丁香蘑菇野生菌株为试材,采用单因素试验和正交实验,研究了其生物学特性及驯化栽培条件,以达到野生丁香蘑菇驯化栽培出菇的目的。结果表明:单因素试验中温度25℃、pH 6、碳源蔗糖、氮源酵母粉、生长因子蘑菇汁,无机盐为硫酸镁,有机物固体培养基为麦粒+牛粪是最佳条件;正交实验表明,4种因子对丁香蘑菇菌株影响程度不同,生长因子>氮源>碳源>无机盐,各因素之间存在显著性差异;出菇栽培试验,分别将草炭土(主料)与羊粪、牛粪、发酵料、草木灰等按3∶1配比,菌丝生长时最适温度20～25℃,pH 6.5～7.5,出菇时最适温度17.5～22.0℃,湿度90%～95%。

The biological characteristics and domestication cultivation of the strain of(Agaricus padanus Lancon.)were studied,which isolated from the wild fruit body that collected from Wushenqi,Ordos city,Inner Mongolia.The optimal conditions of the mycelial growth were determined by single-factor experiment and orthogonal experiment.The results showed that the fungus grew better at 25 ℃,pH 6,sucrose as carbon source,yeast powder as nitrogen source,mushroom juice as growth factor,MgSO4 as inorganic salt,wheat+cow dung as organic(matter)material medium.The orthogonal test showed that the distinct influence of the four factors,growth factor>nitrogen source>carbon source>inorganic salt,and the significant difference among all four factors.Turfy soil was chosen on the domestication cultivation test,3∶1 as the ratio of peat soil mixtured with several different raw materials respectively,such as sheep dung,cow dung,fermentation material and plant ash,20-25 ℃,pH 6.5-7.5 for mycelial growth,17.5-22.0 ℃,the humidity as 90%-95% for fruiting.