黄柏碱对细菌性阴道病小鼠模型的保护作用

Protective effect of phellodendrine on bacterial vaginosis mice

目的探讨黄柏碱改善实验性细菌性阴道病小鼠的作用及机制。方法采用大肠杆菌-金黄色葡萄球菌混合液制备细菌性阴道病模型。ig不同剂量黄柏碱(10、20、40 mg/kg)进行干预治疗,对照组、模型组每日ig等量生理盐水。7 d后观察阴道黏膜病理学变化,ELISA法检测阴道分泌物中白细胞介素-1β(IL-1β)、IL-6、IL-8、肿瘤坏死因子-α(TNF-α)含量及子宫组织中前列腺素E2(PGE2)、前列腺素F2α(PGF2α)水平,Western boltting法检测组织中基质金属蛋白酶-9(MMP-9)、Toll样受体4(TLR4)及核转录因子-κB(NF-κB)蛋白表达水平。结果黄柏碱可有效减轻模型小鼠阴道黏膜病变,显著降低模型小鼠阴道中IL-1β、IL-6、IL-8及TNF-α等炎症因子含量,下调子宫组织中PGE2、PGF2α水平及MMP-9、TLR4、NF-κB蛋白表达水平。结论黄柏碱可有效改善细菌性阴道炎模型小鼠阴道炎症,同时抑制TLR4/NF-κB信号通路,从而改善细菌性阴道病及相应早产风险。

Objective To investigate the impact of phellodendrine on treating bacterial vaginosis(BV) mice. Methods BV model mice were induced by vaginal injecting with EPEC and MRSA. After the mice in control and model groups being administrated with phellodendrine(10, 20, and 40 mg/kg) for 7 d, the mice were sacrificed under deep anesthesia. The mice vaginal pathology changes were observed using HE staining method. IL-1β, IL-6, IL-8, and TNF-α secretion in vagina and PGE2, PGF2α levels in uterus were detected using ELISA assay. Uterine MMP-9, TLR4, and NF-κB expression levels were measured using western blotting. Results Vaginal pathology changes were improved by phellodendrine. Specifically, phellodendrine could reduce IL-1β, IL-6, IL-8, and TNF-α secretion in vagina, it could also reduce PGE2 and PGF2α content, and inhibit MMP-9, TLR4, and NF-κB expression levels in uterus tissue. Conclusion Phellodendrine could effectively treat BV and reduce the rise of pre-term birth by simultaneously inhibiting endovaginal inflammatory response and regulating intrauterine TLR4/NF-κB signal pathway in BV mice.