黄丝郁金挥发油含量测定及指纹图谱的建立

Study on content and fingerprint of volatile oil from Curcuma Longa Radix

目的比较18批黄丝郁金挥发油含量及指纹图谱,为黄丝郁金药材质量控制标准提供依据。方法采用水蒸气蒸馏法提取并测定挥发油含量,同时采用气相色谱(GC)法建立了黄丝郁金挥发油指纹图谱,并用气相色谱-质谱联用(GC-MS)技术确定指纹图谱特征峰,采用相似度评价、聚类分析、主成分分析3种方法对18批黄丝郁金药材指纹图谱进行研究。结果建立了黄丝郁金药材的GC指纹图谱,确定了22个共有峰;通过相似度评价、聚类分析和主成分分析结果可知不同批次黄丝郁金的指纹图谱既有相似性,又有其特征性,不同产区间和同一产区内药材均有一定差异。结论该方法为黄丝郁金药材的质量控制提供了较为全面、有效的评价方法。

Objective To compare and analyze the volatile oil content and fingerprint of 18 batches of Curcuma Longa Radix, so as to provide evidence for its quality control. Methods The content of volatile oil was extracted and determined by steam distillation. At the same time, the fingerprint of the volatile oil of Curcuma Longa Radix was established by GC, and the characteristic peaks of the fingerprint were determined by GC-MS. Similarity evaluation, hierarchical clustering analysis, and principal component analysis was used to investigate fingerprints of 18 batches of Curcuma Longa Radix. Results GC fingerprint of Curcuma Longa Radix was established and 22 common peaks were designated. The results of similarity evaluation, hierarchical clustering analysis and principal component analysis showed that the fingerprints of different batches of Curcuma Longa Radix had similarities and characteristics, and there were some differences among different habitats and the same origin. Conclusion This study provides a comprehensive and effective evaluation method for the quality control of Curcuma Longa Radix.