续断“发汗”前后水煎液及含药血清对人成骨样MG-63细胞、幼鼠成骨细胞增殖的影响

Effect of decoction and drug-containing serum of sweated and crude Radix Dipsaci on proliferation of MG-63 cells and osteoblasts

目的探讨续断"发汗"前后水煎液及含药血清对人成骨样MG-63细胞、成骨细胞增殖的影响。方法将人成骨样MG-63细胞、幼鼠成骨细胞分别与续断"发汗"前后水煎液及大鼠含药血清共同培养。MTT法检测细胞增殖,硝基苯磷酸盐法检测成骨细胞的碱性磷酸酶(ALP)活性。结果续断水煎液和含药血清均能显著促进MG-63细胞和成骨细胞增殖(P<0.01),并能显著提高成骨细胞的ALP活性(P<0.01),且相同剂量未"发汗"组作用普遍优于或非劣于"发汗"组。结论结合前期成分研究,推断续断"发汗"前后成分的改变影响了其促进细胞增殖和分化的作用,为其进一步研究奠定基础。

Objective To investigate the effect of decoction and drug serum of sweated and crude Dipsaci Radix on the proliferation of human osteoblast-like cells(MG-63) and osteoblasts. Methods MG-63 cells and osteoblasts were co-cultured with decoction and rat serum containing Dipsaci Radix before and after "sweating". The cell proliferation was detected by MTT method and the ALP activity of osteoblasts was detected by nitrophenyl phosphate method. Results Both decoction and drug-containing serum can significantly promote the proliferation of MG-63 cells and osteoblasts(P < 0.01), and significantly increase the ALP activity of osteoblasts(P < 0.01). Moreover, sweated group were generally better than or non-inferior to crude group at the same dose concentration of two administration methods. Conclusion Combined with component studies, it can be inferred that the changes in composition before and after "sweating" affect its role in promoting cell proliferation and differentiation, with view to laying the foundation for further research.