外引玉米种质对两种穗腐病原镰孢菌抗性鉴定

Test for Ear Rot Resistance Against Fusarium verticillioides and Fusarium graminearum in Imported Maize Germplasm

采用双牙签接种技术,2016-2017年连续2年对从美国、加拿大、罗马尼亚、德国、法国、荷兰等国家引进的177份玉米种质资源抗玉米穗腐病原拟轮枝镰孢(F.verticillioides)和禾谷镰孢(F.graminearum)进行了鉴定。结果表明,鉴定的177份玉米种质资源中,对拟轮枝镰孢表现高抗(HR)的资源2份,占总数的1.13%;抗病(R)38份,占总数的21.47%;中抗(MR)75份,占总数的42.37%;感病(S)48份,占总数的27.12%;高感(HS)14份,占总数的7.91%。177份玉米种质资源中,对禾谷镰孢(F.graminearum)表现抗病(R)12份,占鉴定资源总数的6.78%;中抗(MR)66份,占总数的37.29%;感病(S)64份,占总数的36.16%;高感(HS)35份,占总数的19.77%,未发现对禾谷镰孢菌表现高抗的材料。在177份玉米种质资源中,兼抗两种穗腐病原镰孢菌的种质资源有12份。

A total of 177 maize germplasm accessions,which were imported from the countries of USA,Canada,Romania,Germany,France and Netherlands,were evaluated for resistance against ear rot causing agent of Fusarium verticillioides(FV)and Fusarium graminearum(FG)with double toothpick inoculation technique in two calendar years(2016-2017).Out of that,two accessions(1.13% of total tested accessions)were highly resistant(HR)to FV,and 38 accessions(21.47%)were resistant(R).75,48 and 14 accessions showed moderate resistance(MR,42.37%),susceptible(S,27.12%)and highly susceptible(HS,7.91%),respectively.Upon inoculation of FG,12 and 66 accessions were resistant(R,6.78%)and moderately resistant(MR,37.29%),respectively.Meanwhile,64 and 35 accessions were susceptible(S,36.16%)and highly susceptible(HS,19.77%),respectively.No accessions with highly resistant(HR)to FG was found.Attractively,twelve accessions with resistant to both FV and FG were identified,and they might serve as resistance resource used in maize breeding for ear rot resistance.