摘要
为明确鸭源多杀性巴氏杆菌对磺胺类药物的耐药性及耐药基因的分布特征,试验采用琼脂二倍稀释法对分离自福建、广东和江西等地的89株鸭源多杀性巴氏杆菌进行甲氧苄啶/磺胺甲噁唑的耐受性检测,用PCR法检测各耐药菌株的磺胺类耐药基因(sul1、sul2和sul3),并利用多位点序列分型(MLST)分析耐药菌株间的亲缘关系。结果显示,33株鸭源多杀性巴氏杆菌对甲氧苄啶/磺胺甲噁唑耐受,耐药率为37.1%(33/89);在耐药菌株中,sul1、sul2和sul3基因检出率分别为100%(33/33)、97.0%(32/33)和21.2%(7/33),其中sul1和sul2基因同时检出率为97.0%(32/33),sul1、sul2和sul3基因同时检出率为21.2%(7/33);所有耐药菌株均属于ST129型。以上结果表明,鸭源多杀性巴氏杆菌对磺胺类药物具有一定的耐药性,其中的耐药菌株都属于同一序列型ST129,均携带有sul1耐药基因,且绝大多数耐药菌株同时携带sul1与sul2两种耐药基因,提示在福建、广东和江西等地需慎用磺胺类药物来防治该细菌的感染。
In order to understand the antimicrobial sensitibity to sulfonamides and distribution ofsulfonamides resistance genes of Pasteurella multocida strains isolated from ducks in Fujian,Guangdong,Jiangxi,etc,89 Pasteurella multocida strains were tested for antimicrobial susceptibility to trimethoprim-sulfamethoxazole by agar dilution method,PCR method and multi-locus sequence typing(MLST)were used for detection of sulfonamides resistance genes and to analyze the genetic relationship among the sulfonamides-resistant strains.The results showed that 33strains(37.1%)were resistant to trimethoprim-sulfamethoxazole.Among the 33 sulfonamidesresistant strains,the detection rates of sul1,sul2 and sul3genes were 100%(33/33),97.0%(32/33)and 21.2%(7/33).The simultaneous detection rate of sul1 and sul2genes were 97.0%(32/33).The simultaneous detection rate of sul1,sul2 and sul3genes were 21.2%(7/33).All of the33sulfonamides-resistant strains belonged to the ST129 sequence type.The results indicated that there was sulfonamides resistance to Pasteurella multocidaisolated from ducks,and all the sulfonamides-resistant strains,which belonged to the same sequence type ST129,carried sul1 resistant gene,most of sulfonamides-resistant strains carried sul1 and sul2resistant genes,suggesting that it should be rationally used sulfonamides to prevent and cure the infection of Pasteurella multocidaisolated from ducks in some areas of Fujian,Guangdong and Jiangxi,etc.
作者
陈红梅
程龙飞
邓辉
刘荣昌
傅光华
施少华
傅秋玲
万春和
郑庆礼
黄瑜
黄小红
CHEN Hongmei;CHENG Longfei;DENG Hui;LIU Rongchang;FU Guanghua;SHI Shaohua;FU Qiuling;WAN Chunhe;ZHENG Qingli;HUANG Yu;HUANG Xiaohong(College of Life Science,Fujian Agriculture and Forestry University,Fuzhou 350002,China;Fujian Animal Diseases Control Technology Center,Fujian Provincial Key Laboratory for Avian Diseases Control and Prevention,Institute of Animal Husbandry and Veterinary Medicine,Fujian Academy of Agricultural Sciences,Fuzhou 350013,China;University Key Laboratory for Integrated Chinese Traditional and Western Veterinary Medicine and Animal Healthcare in Fujian Province,College of Animal Science and Technology,Fujian Agriculture and Forestry University,Fuzhou 350002,China)
出处
《中国畜牧兽医》
CAS
北大核心
2019年第1期223-230,共8页
China Animal Husbandry & Veterinary Medicine
基金
现代农业产业技术体系建设专项资金(CARS-42-18)
福建省属公益类科研院所基本科研项目(2016R1022-2)
福建省农业科学院畜牧兽医创新团队(STIT2017-1-5)
福建省教育厅实验室开放基金项目
