鹿角盘水提物对大鼠乳腺增生的作用效果及其机制研究

Effects of Deer Hard Antler Buttons Water Extracts on Hyperplasia of Mammary Glands in Rats and Underlying Mechanism

为了探索鹿角盘(DHAB)水提物对大鼠乳腺增生的治疗作用,初步探讨其作用机理和用药剂量,试验利用苯甲酸雌二醇(0.5mg/(kg·d))联合黄体酮(5.0mg/(kg·d))对SD大鼠进行肌内注射建立乳腺增生模型。试验共分7个组,每组10只大鼠,分别为空白组、乳腺增生症(HMG)模型组、阳性药三苯氧胺组(0.036mg/(kg·d))、鹿角盘水提物4个剂量组(高剂量,2.625g/(kg·d);中高剂量,1.575g/(kg·d);中低剂量,1.05g/(kg·d);低剂量,0.63g/(kg·d))。按组别连续灌胃45d,每周固定时间测定大鼠乳头直径、高度及体重。试验结束后采血取样,称量大鼠胸腺、脾脏、子宫和卵巢质量,取乳腺组织做病理学切片、HE染色,用放射免疫法检测大鼠血清中雌二醇(E2)及孕酮(P)的含量。结果表明,使用不同剂量鹿角盘水提物均能减小乳腺增生大鼠乳头直径、高度,提高胸腺、脾脏指数,降低子宫指数,减轻乳腺小叶数、腺泡数和分泌物,降低血清E2含量,升高血清P的含量,与模型组相比有统计学差异(P<0.01;P<0.05))。综合试验结果,鹿角盘水提物对乳腺增生大鼠有治疗作用,效果显著,且以中高剂量组变化最为明显。其作用机制可能通过增强机体免疫力、调节血清中E2、P水平实现。

To explore the therapeutic effects of deer hard antler button(DHAB)water extracts on hyperplasia of mammary glands(HMG)in rats and to explore its mechanism of action and dosage,the model of HMG was established through intramuscular injection of estradiol benzoate(0.5mg/(kg·d))and progesterone(5.0mg/(kg·d))in 70 SD rats.There were 7groups with10 rats in each group,which were divided into blank group,HMG model group,positive drug tamoxifen group(0.036mg/(kg·d)),4DHAB groups(high-dose,H,2.625g/(kg·d);Medium-high-dose,MH,1.575g/(kg·d);Medium-low-dose,ML,1.05g/(kg·d);Low-dose,L,0.63g/(kg·d)).Each group was treated with intragastric administration for 45 d.The diameter and height of nipple and body weight were recorded weekly.Blood samples were taken and thymus,spleen,uterus and ovary weights of the rat breasts were measured at the end of the test.Histopathological sections and HE staining were performed and sex hormone estradiol(E2)and progesterone(P)were detected by radioimmunoassay.The results indicated that different doses of DHAB water extracts could reduce the diameter and height of nipple,increase thymus and spleen indexes and reduce uterine index,reduce the number of breast lobules,acinar numbers and secretion contents,and decrease E2 and increase P levels in serum of HMG rats.All these indexes were significantly or extremely significantly different from the model group(P<0.01;P<0.05).In conclusion,DHAB water extracts had significant therapeutic effects on HMG in rats,and the medium-high-dose was the best.The action mechanism of DHAB water extracts was enhancing immunity and regulating serum E2 and P levels.