淫羊藿苷对高糖环境下大鼠颌骨骨髓基质细胞信号通路Shh的影响

Effects of Shh on bone marrow stromal cells in rat bone marrow stromal cells induced by high glucose

目的探讨淫羊藿苷对高糖环境下大鼠颌骨骨髓基质细胞成骨相关信号通路Shh的影响。方法将传代培养的大鼠颌骨骨髓基质细胞分为4组:正常组(2%胎牛血清+DMEM培养的细胞),高糖组(2%胎牛血清+高糖-DMEM培养的细胞),淫羊藿苷组1(2%胎牛血清+DMEM+淫羊藿苷培养的细胞),淫羊藿苷组2(2%胎牛血清+高糖-DMEM+淫羊藿苷培养的细胞),荧光定量检测Shh通路相关基因PTCH1和SMO的表达情况,免疫荧光检测核心蛋白Gli-1蛋白表达情况。结果淫羊藿苷组1与其它组比较:通路相关基因PTCH1和SMO的表达均高于对照组;免疫荧光检测显示核通路心蛋白Gli-1表达增强。淫羊藿苷组2与高糖组比较,各项检测也均有显著性差异(P<0.05)。结论淫羊藿苷能提高高糖培养条件下大鼠颌骨骨髓基质细胞Shh信号通路激活水平,细胞成骨能力增加。

Objective To investigate the effect of the content of Shh on the osteogenic signaling pathway of bone marrow stromal cells of rat bone marrow stromal cells under high glucose environment.Methods Stromal cells were divided into four groups:normal group(2% fetal bovine serum + DMEM cultured cells),high glucose group(2% fetal bovine serum + high glucose-DMEM cultured cells),icariin group 1(2% fetal bovine serum + DMEM + icariin cultured cells),and icariin group 2(2% fetal bovine serum + high glucose-DMEM + icariin cultured cells).The expression of PTCH 1 and SMO genes related to Shh pathway were detected by quantitative fluorescence and the expression of Gli-1 protein was detected by immunofluorescence.Results Compared with other groups,the expression of PTCH1 and SMO in the pathway related genes was higher than that in the control group,the expression of cardiac protein Gli-1 was enhanced in the nuclear pathway by immunofluorescence assay.Compared with the high glucose group,there were significant differences between the 2 groups(P<0.05).Conclusion The level of Shh signaling pathway activation in rat bone marrow stromal cells can be increased by high glucose culture.