24位点可变串联重复序列和间隔区寡核苷酸分型对河南省结核分枝杆菌基因型的鉴定

Genotypic analysis of Mycobacterium tuberculosis strains collected from He′nan Province by 24-locus variable-number tandem-repeat typing and spoligotyping

目的了解河南省结核分枝杆菌的基因型别。方法采用经典24位点分枝杆菌散在重复单元-可变串联重复序列(mycobacterium interspersed repetitive unit -variable number of tandem repeat,MIRU-VNTR)和间隔区寡核苷酸分型(spoligotyping)对2015年间河南省不同地区的668株结核分枝杆菌进行基因分型。结果间隔区寡核苷酸分型将668株菌分为11个家族,35个基因型。在558株北京家族基因型中,典型北京家族菌株546株,非典型北京家族菌株12株。在110株非北京家族菌株中,8株为新发现菌株;另102株非北京家族菌株被分为10个家族,其中T家族有76株,包括T1家族59株,T2家族7株,T3家族10株。标准24位点VNTR将668株菌分为550个基因型,有508株菌显示出单一的基因型,另160株菌被分到42个不同的基因簇中。最大簇含有21株菌,其余簇包含2~20株菌。结论河南省结核分枝杆菌主要为北京家族基因型。

Objective To investigate the genotype of M.tuberculosis in He′nan Province. Methods A total of 668 M.tuberculosis clinical strains collected in difference regions of He′nan Province during 2015 were genotyped by two standard methods, including classical 24-locus mycobacterium interspersed repetitive unit variable-number tandem-repeat (MIRU-VNTR) typing and spoligotyping. Results The 668 isolates were divided into 11 clusters and 35 patterns by spoligotyping.Among the 558 Beijing strains, 546 were typical Beijing strains and the other 12 were atypical Beijing strains.Among the 110 non-Beijing strains, eight were new strains and the remaining 102 non-Beijing strains were divided into 10 families.There were 76 isolates belonging to T family, including 59 of T1 families, 7 of T2 families, and 10 of T3 families.The 668 strains were divided into 550 gene patterns by standard 24-locus VNTR, including 508 un-clustered patterns and 160 clustered into 42 clusters.The largest cluster contained 21 strains, the other clusters contained 2-20 strains. Conclusion Beijing strain is still the most prevalent M.tuberculosis in He′nan Province.